- Extracellular Vesicle-Mediated Modulation of Stem-like Phenotype in Breast Cancer Cells under Fluid Shear Stress

Verification of isolation of extracellular vesicles (EVs) produced by serum-free MDA-MB-231 cells. (A) Representative top surface scanning electron micrograph (FE-SEM) of a pre-filtration Synder LY membrane used for EV isolation (left; scale bar: 100 nm) and histogram demonstrating size distribution of pore sizes (right). (B) Representative transmission electron micrograph (TEM) image of EVs derived using ultracentrifugation (UC; left) and of EVs derived using direct flow filtration (DFF; right). (C) Exosome Antibody Array of MDA-MB-231 whole cell lysate, UC-derived EV proteins, and DFF-derived EV proteins (50 µg each). Numbers represent relative intensity of the bands compared to the positive control. Original Western blot images are available in Supplementary Materials. (D) Total protein isolated from EVs per one million cells. n ≥ 3; mean ± standard error; *** p < 0.001 via Student’s t-test with Welch corrections.

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Extracellular Vesicle-Mediated Modulation of Stem-like Phenotype in Breast Cancer Cells under Fluid Shear Stress - Scientific Figure on ResearchGate. Available from: https://www.researchgate.net/figure/Verification-of-isolation-of-extracellular-vesicles-EVs-produced-by-serum-free_fig1_381779636 [accessed 22 May 2025]

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Verification of isolation of extracellular vesicles (EVs) produced by serum-free MDA-MB-231 cells. (A) Representative top surface scanning electron micrograph (FE-SEM) of a pre-filtration Synder LY membrane used for EV isolation (left; scale bar: 100 nm) and histogram demonstrating size distribution of pore sizes (right). (B) Representative transmission electron micrograph (TEM) image of EVs derived using ultracentrifugation (UC; left) and of EVs derived using direct flow filtration (DFF; right). (C) Exosome Antibody Array of MDA-MB-231 whole cell lysate, UC-derived EV proteins, and DFF-derived EV proteins (50 µg each). Numbers represent relative intensity of the bands compared to the positive control. Original Western blot images are available in Supplementary Materials. (D) Total protein isolated from EVs per one million cells. n ≥ 3; mean ± standard error; *** p < 0.001 via Student’s t-test with Welch corrections.

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<a href="https://www.researchgate.net/figure/Verification-of-isolation-of-extracellular-vesicles-EVs-produced-by-serum-free_fig1_381779636"><img src="https://www.researchgate.net/publication/381779636/figure/fig1/AS:11431281376821778@1744673788772/Verification-of-isolation-of-extracellular-vesicles-EVs-produced-by-serum-free.tif" alt="Verification of isolation of extracellular vesicles (EVs) produced by serum-free MDA-MB-231 cells. (A) Representative top surface scanning electron micrograph (FE-SEM) of a pre-filtration Synder LY membrane used for EV isolation (left; scale bar: 100 nm) and histogram demonstrating size distribution of pore sizes (right). (B) Representative transmission electron micrograph (TEM) image of EVs derived using ultracentrifugation (UC; left) and of EVs derived using direct flow filtration (DFF; right). (C) Exosome Antibody Array of MDA-MB-231 whole cell lysate, UC-derived EV proteins, and DFF-derived EV proteins (50 µg each). Numbers represent relative intensity of the bands compared to the positive control. Original Western blot images are available in Supplementary Materials. (D) Total protein isolated from EVs per one million cells. n ≥ 3; mean ± standard error; *** p < 0.001 via Student’s t-test with Welch corrections."/></a>