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Tropism of 10 populations of Radopholus similis to the Citrus limon root. A: Tropism of R. similis to the root of C. limon in Pluronic F-127 gel evenly mixed with 200 female nematodes 6 h after assay initiation; B: Fuchsin staining of C. limon roots 8 h after assay initiation by infection with 200 female nematodes; XIN, ML, SWK, HN6, HL, GJ, FZ, GZ, YJ, and DBSR: the C. limon roots were infected with populations of R. similis, which originated from Zingiber officinale Roscoe (XIN), Crataegus pinnatifida (ML), Musa AAA Giant Cavendish cv.Baxi (HN6), Maranta arundinacea (HL), Chrysalidocarpus lutescens (SWK), Citrus reticulata (GJ), Anthurium andraeanum ‘Pink Champion’ (FZ), Anthurium andraeanum Linden (GZ), Curcuma longa (YJ), and Anubias nana (DBSR), respectively; scale bar = 500 μm

Tropism of 10 populations of Radopholus similis to the Citrus limon root. A: Tropism of R. similis to the root of C. limon in Pluronic F-127 gel evenly mixed with 200 female nematodes 6 h after assay initiation; B: Fuchsin staining of C. limon roots 8 h after assay initiation by infection with 200 female nematodes; XIN, ML, SWK, HN6, HL, GJ, FZ, GZ, YJ, and DBSR: the C. limon roots were infected with populations of R. similis, which originated from Zingiber officinale Roscoe (XIN), Crataegus pinnatifida (ML), Musa AAA Giant Cavendish cv.Baxi (HN6), Maranta arundinacea (HL), Chrysalidocarpus lutescens (SWK), Citrus reticulata (GJ), Anthurium andraeanum ‘Pink Champion’ (FZ), Anthurium andraeanum Linden (GZ), Curcuma longa (YJ), and Anubias nana (DBSR), respectively; scale bar = 500 μm

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Radopholus similis is a destructive, migratory, and endophytoparasitic nematode. It has two morphologically indistinguishable pathotypes (or physiological races): banana and citrus pathotypes. At present, the only reliable method to differentiate the two pathotypes is testing the infestation and parasitism of nematodes on Citrus spp. via inoculatio...

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