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Figure 1 - Pronounced limb and fibre type differences in subcellular lipid droplet content and distribution in elite skiers before and after exhaustive exercise

Figure 1. Transmission electron microscopy and point-counting of subcellular lipid droplets A, acquisition of electron micrographs was systematically randomized to cover the subsarcolemmal (SS), superficial myofibrillar (SMF) and central myofibrillar regions (CMF) of each longitudinally oriented fibre. The arrow indicates the sarcolemma. (original magnification ×600, scale bar: 20 µm). B, subcellular localisations of lipid droplets. The arrow indicates the sarcolemma. C, close-up view of point-counting of subsarcolemmal lipid droplet volume fraction with grid (size 135 nm) overlay. The open circles indicate intersections touching a lipid droplet. * , a subsarcolemmal lipid droplet; * * , an intermyofibrillar lipid droplet; Mi, mitochondria; Z, Z-line. (Original magnification ×10,000, scale bar: 1 µm.)
Transmission electron microscopy and point-counting of subcellular lipid droplets A, acquisition of electron micrographs was systematically randomized to cover the subsarcolemmal (SS), superficial myofibrillar (SMF) and central myofibrillar regions (CMF) of each longitudinally oriented fibre. The arrow indicates the sarcolemma. (original magnification ×600, scale bar: 20 µm). B, subcellular localisations of lipid droplets. The arrow indicates the sarcolemma. C, close-up view of point-counting of subsarcolemmal lipid droplet volume fraction with grid (size 135 nm) overlay. The open circles indicate intersections touching a lipid droplet. * , a subsarcolemmal lipid droplet; * * , an intermyofibrillar lipid droplet; Mi, mitochondria; Z, Z-line. (Original magnification ×10,000, scale bar: 1 µm.)
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