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Transformed hairy roots formation on Capsicum annuum seedlings (sweet variety) inoculated via Agrobacterium rhizogenes 1601. a. Hairy roots formed on pepper seedlings at injection sites (10 days old) on solidified MS medium (arrowed). b. Hairy roots developed on seedlings (two weeks old). c. Culture of hairy roots having dense root hairs on solidified MS medium (6 weeks old).

Transformed hairy roots formation on Capsicum annuum seedlings (sweet variety) inoculated via Agrobacterium rhizogenes 1601. a. Hairy roots formed on pepper seedlings at injection sites (10 days old) on solidified MS medium (arrowed). b. Hairy roots developed on seedlings (two weeks old). c. Culture of hairy roots having dense root hairs on solidified MS medium (6 weeks old).

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Genetic transformation using Agrobacterium is one of the techniques used to transfer desired genes to plants. This protocol is considered a shortcut to get transformed plants which could be an alternative method and suitable system compared with the classical methods. This study aimed to investigate the response of C. annuum seedlings (sweet variet...

Contexts in source publication

Context 1
... biological interaction process was succeeded between A. rhizogenes R1601 and C. annuum seedlings. Hairy roots were developed from injected sites on seedlings after 10 days (Figure 1, a). The number hairy roots formed on each seedling was 2-5 roots. ...
Context 2
... number hairy roots formed on each seedling was 2-5 roots. After sequential transfer of these roots on solid MS medium they were obtained after two weeks (Figure 1, b). These adventitious hairy roots were negatively geotropism in their growth with dense of root hairs (Figure 1, c). ...
Context 3
... sequential transfer of these roots on solid MS medium they were obtained after two weeks (Figure 1, b). These adventitious hairy roots were negatively geotropism in their growth with dense of root hairs (Figure 1, c). Moreover, the responses of seedlings to inoculation by A. rhizogenes was 90% compared with the control samples that did not show any response (Table 1). ...