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- Expression and localization of myosin VI in developing mouse spermatids

Toluidine blue staining (a–g) and immunofluorescence labeling of MVI (h) and actin (i) of mouse seminiferous tubules during spermatogenis. aSpT spermatid at the acrosome phase, BV blood vessel, gSpT spermatid at the Golgi phase, Lc Leydig cell, mSpT spermatid at the maturation phase, Sc Sertoli cell, SE seminiferous epithelium, SpC spermatocyte, SpG spermatogonium, SpZ spermatozoa, STL seminiferous tubule lumen. Arrows or double arrows show MVI (red) and actin (green) staining in spermatids at maturation or acrosome phase, respectively; stars in i show actin localization in basal ectoplasmic specialization; dashed lines basement membrane. Nuclei are stained with DAPI (blue). Bars 50 μm (a), 20 μm (g–i), 5 μm (b-f)
Toluidine blue staining (a–g) and immunofluorescence labeling of MVI (h) and actin (i) of mouse seminiferous tubules during spermatogenis. aSpT spermatid at the acrosome phase, BV blood vessel, gSpT spermatid at the Golgi phase, Lc Leydig cell, mSpT spermatid at the maturation phase, Sc Sertoli cell, SE seminiferous epithelium, SpC spermatocyte, SpG spermatogonium, SpZ spermatozoa, STL seminiferous tubule lumen. Arrows or double arrows show MVI (red) and actin (green) staining in spermatids at maturation or acrosome phase, respectively; stars in i show actin localization in basal ectoplasmic specialization; dashed lines basement membrane. Nuclei are stained with DAPI (blue). Bars 50 μm (a), 20 μm (g–i), 5 μm (b-f)
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