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The response of the average treated isolates (Granulicatella adiacens, Staphylococcus sciuri and Kocuria spp.) towards the average concentrations of aqueous bark extract of Juglans regia L.. The error bars represent ± standard deviation (SD). Bars with different letters demonstrate the significant differences (ANOVA, one way, Tukey, P<0.05, n=7).
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Juglans regia is one of the medicinal plants widely applied in many applications due to its pharmacological value and desirable characteristics of its parts. Hence, there is a motivation of medicinal and cosmetics applications. In this paper, the crude aqueous extracts from Juglan regia bark were screened for in vitro antibacterial properties again...
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... It has been accepted that herbs and medicinal plants have antimicrobial activity against pathogenic bacteria, and antioxidant activity [7-9-10]. due to their contents of bioactive natural compounds [11]. There has been a special emphasis on the application of natural food additives. ...
The aim: It has been well established that thyme has antioxidant activity and microbial inhibition capacity against different pathogens. Hence this study was conducted to investigate the effect of adding thyme (aqueous and alcoholic) extract on beef mincemeat quality. Materials and methods: Three different concentrations (0.4, 0.8.and 1.2 mg/ml) were used. Beef mincemeat stored at 4C° for 7 and 14 days after mixing with the extracts. The meat samples not treated with the extracts are considered as a control group. Different bacteriological indicators e.g. (Total plate count, Total coliform, Salmonella and Shigella count, and Staphylococcus aureus) were assessed to validate the efficiency of the extract to prolonged the storage period at 4C°. Results: The results of the antimicrobial assay of (aqueous and alcoholic extracts of thyme at different concentrations showed that the aqueous extract had significant inhibitory action on the growth of a wide range of bacteria compared to the alcoholic extract. Conclusions: Thus, the thyme aqueous extracts can be efficient and promising as a preservative for the meat and its products especially at high concentrations to inhibit bacterial growth.
... (2) The presence of E. gingivalis in dental biofilms of patients with periodontal disease and in the control group subjects validates previous hypotheses of studies that this protozoan may contribute to the progression of periodontal disease. (2,4) This parasite may contribute to periodontitis development by assisting the bacteria that cause damage (3,10) or by invading ruptured oral mucosa and ingesting fragments of live cells, as would be required to justify its periodontal pathogenicity. (4) In terms of gender, E. gingivalis was found in more males (59.09%) than females (40.91%). ...
Background: In addition to human cells, healthy gums contain a variety of bacteria, fungi, and protozoa that may contribute to unhealthy sites. The effect of pathogens lysis or other immune cell lysis needs to be clarified in order to expand the current pathophysiological pattern of periodontitis. The purpose of this study was to survey the prevalence of E. gingivalis among patients with periodontitis and healthy individuals in order to support public health action. In addition, we investigated the relationship between the incidence rate and some parameters, such as patient age and gender. Methods and Results: This study enrolled 51 subjects divided into two groups: The case group involved 31 patients (15 females and 16 males) with periodontal disease; the control group included 20 people (10 females and 10 males) with healthy oral cavities. Biofilm and saliva samples were collected and examined for the presence of E. gingivalis. A total of 22(71.0%) patients of various ages with periodontal disease were infected with E. gingivalis. Male patients were more likely to have the amoeba than female patients. E. gingivalis was also found in the control group, albeit at a lower frequency (P=0.005). Conclusion: Infections with E. gingivalis should be considered as a potential pathological factor associated with the development of periodontal diseases. More research should be conducted to determine whether E. gingivalis is the primary cause of tissue destruction and disease progression, or if it is acting synergistically with the current pathogenic bacteria that should be investigated further.(International Journal of Biomedicine. 2022;12(3):409-411.).
... (2) The presence of E. gingivalis in dental biofilms of patients with periodontal disease and in the control group subjects validates previous hypotheses of studies that this protozoan may contribute to the progression of periodontal disease. (2,4) This parasite may contribute to periodontitis development by assisting the bacteria that cause damage (3,10) or by invading ruptured oral mucosa and ingesting fragments of live cells, as would be required to justify its periodontal pathogenicity. (4) In terms of gender, E. gingivalis was found in more males (59.09%) than females (40.91%). ...
Background: In addition to human cells, healthy gums contain a variety of bacteria, fungi, and protozoa that may contribute to unhealthy sites. The effect of pathogens lysis or other immune cell lysis needs to be clarified in order to expand the current pathophysiological pattern of periodontitis. The purpose of this study was to survey the prevalence of E. gingivalis among patients with periodontitis and healthy individuals in order to support public health action. In addition, we investigated the relationship between the incidence rate and some parameters, such as patient age and gender. Methods and Results: This study enrolled 51 subjects divided into two groups: The case group involved 31 patients (15 females and 16 males) with periodontal disease; the control group included 20 people (10 females and 10 males) with healthy oral cavities. Biofilm and saliva samples were collected and examined for the presence of E. gingivalis. A total of 22(71.0%) patients of various ages with periodontal disease were infected with E. gingivalis. Male patients were more likely to have the amoeba than female patients. E. gingivalis was also found in the control group, albeit at a lower frequency (P=0.005). Conclusion: Infections with E. gingivalis should be considered as a potential pathological factor associated with the development of periodontal diseases. More research should be conducted to determine whether E. gingivalis is the primary cause of tissue destruction and disease progression, or if it is acting synergistically with the current pathogenic bacteria that should be investigated further.
... Chitin is the second most prevalent polysaccharide in nature after cellulose and is made from (-(1-4)-poly-N-acetyl-D-glucosamine). Fungal spore germination, hyphal elongation, and radial growth are well documented to be inhibited by chitosan (3). ...
... A higher electrostatic interaction between positively charged chitosan amino groups and negatively charged bacterial surfaces may occur in gram-negative bacteria (24). Gram-negative bacteria have higher hydrophilicity and chitosan adsorption on their cell walls than those of gram-positive bacteria, which may contribute to the A.B. effect (3,21). Also, the structural arrangement of envelope/membrane components in gram-positive and gram-negative organisms. ...
Chitin is the most substantial natural polysaccharide after cellulose, found in the shells of crabs, shrimps, and
other crustaceans. Several medical and environmental applications have been recognized for chitosan.
Therefore, the present study aimed to evaluate the biological activity of laboratory-prepared chitosan from
shrimp shells against pathogenic bacteria isolates. In the present study, chitosan was extracted from chitin
acetate of shrimp shells at different temperatures (room temperature, 65 and 100 ° C) for equal amounts of
shells at specified time intervals. The degree of acetylation of different treatments of RT1, RT2, and RT3
reached 71%, 70%, and 65%, respectively. The laboratory-prepared chitosan was examined and antibacterial
properties were observed against clinical isolates of bacterial causative agents of urinary tract infections (E. coli,
Klebsiella Pneumonia, Pseudomonas spp., Citrobacter freundii, and Enterobacter spp.). The inhibitory activity
of all types of treatments ranged between 12 to 25 mm for all isolates with the highest for Enterobacter spp. and
the lowest for Pseudomonas isolates. The results also indicated a large relative discrepancy between the
inhibitory activity of laboratory-prepared chitosan and antibiotics. These results were in the S-R range of the
isolates. The similarity of laboratory production conditions and treatments is due to the different proportions of
chitin formed in shrimp, environmental conditions, nutrition factors, pH, the extent of heavy metals in the water,
and the age of the organism.
... Various researches establish that natural preservatives can be used as an alternative to enhance food quality without altering food taste (Ukrainets 2016). Antimicrobial and antioxidant activities of natural derivatives against food spoiling microbes have been reported by various researchers (Ukrainets 2016;Emeka and Chiamaka 2020;Efenberger-Szmechtyk et al. 2020;Pateiro et al. 2021;Salman et al. 2021a), these antimicrobial and antioxidant activities are associated with the content of bioactive natural compounds that are present in these plants (Salman et al. 2021b). Further, these natural additives have proven their ability and effectiveness to reduce the effects of oxidative damage, delay in the emergence of objectionable flavors, and improve the food status (color of meat dyes) (Efenberger-Szmechtyk et al. 2020). ...
Antioxidant and antimicrobial activity of thyme has been well established against various microorganisms. This study was carried out to investigate the effect of aqueous and alcoholic extract of thyme on beef mincemeat quality. Three differential concentrations (0.4, 0.8, and 1.2 mg/ml) of both thyme extracts were used for the beef mincemeat preservation. Untreated meat samples were considered as the control group while the extracts treated beef mincemeat are stored at 4°C for 7 to 14 days. To validate the extract's ability to prolong the storage period at 4 °C, various bacteriological indicators like total plate count, presence of total coliform, Salmonella, Shigella, and Staphylococcus aureus count were assessed. The results of the antimicrobial assay of aqueous and alcoholic extracts of thyme at different concentrations showed that the aqueous extract had significant inhibitory action on the growth of a wide range of bacteria compared to the alcoholic extract. Thus, the thyme aqueous extracts can be efficient and promising as preservatives for meat and its products, especially at high concentrations to inhibit bacterial growth.
... Various researches establish that natural preservatives can be used as an alternative to enhance food quality without altering food taste (Ukrainets 2016). Antimicrobial and antioxidant activities of natural derivatives against food spoiling microbes have been reported by various researchers (Ukrainets 2016;Emeka and Chiamaka 2020;Efenberger-Szmechtyk et al. 2020;Pateiro et al. 2021;Salman et al. 2021a), these antimicrobial and antioxidant activities are associated with the content of bioactive natural compounds that are present in these plants (Salman et al. 2021b). Further, these natural additives have proven their ability and effectiveness to reduce the effects of oxidative damage, delay in the emergence of objectionable flavors, and improve the food status (color of meat dyes) (Efenberger-Szmechtyk et al. 2020). ...
Antioxidant and antimicrobial activity of thyme has been well established against various microorganisms. This study was carried out to investigate the effect of aqueous and alcoholic extract of thyme on beef mincemeat quality. Three differential concentrations (0.4, 0.8, and 1.2 mg/ml) of both thyme extracts were used for the beef mincemeat preservation. Untreated meat samples were considered as the control group while the extracts treated beef mincemeat are stored at 4°C for 7 to 14 days. To validate the extract's ability to prolong the storage period at 4 °C, various bacteriological indicators like total plate count, presence of total coliform, Salmonella, Shigella, and Staphylococcus aureus count were assessed. The results of the antimicrobial assay of aqueous and alcoholic extracts of thyme at different concentrations showed that the aqueous extract had significant inhibitory action on the growth of a wide range of bacteria compared to the alcoholic extract. Thus, the thyme aqueous extracts can be efficient and promising as preservatives for meat and its products, especially at high concentrations to inhibit bacterial growth.
Periodontal diseases and dental caries are the most common infectious oral diseases impacting oral health globally. Oral cavity health is crucial for enhancing life quality since it serves as the entranceway to general health. The oral microbiome and oral infectious diseases are strongly correlated. Gram-negative anaerobic bacteria have been associated with periodontal diseases. Due to the shortcomings of several antimicrobial medications frequently applied in dentistry, the lack of resources in developing countries, the prevalence of oral inflammatory conditions, and the rise in bacterial antibiotic resistance, there is a need for reliable, efficient, and affordable alternative solutions for the prevention and treatment of periodontal diseases. Several accessible chemical agents can alter the oral microbiota, although these substances also have unfavorable symptoms such as vomiting, diarrhea, and tooth discoloration. Natural phytochemicals generated from plants that have historically been used as medicines are categorized as prospective alternatives due to the ongoing quest for substitute products. This review concentrated on phytochemicals or herbal extracts that impact periodontal diseases by decreasing the formation of dental biofilms and plaques, preventing the proliferation of oral pathogens, and inhibiting bacterial adhesion to surfaces. Investigations examining the effectiveness and safety of plant-based medicines have also been presented, including those conducted over the past decade.
