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The qualitative analysis of endophytic bacteria isolates to solubilize phosphate on Pikovskaya medium with the addition of Bromophenol blue 0.01 g L -1
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Sudewi S, Ala A, Baharuddin, Farid M. 2020. The isolation, characterization endophytic bacteria on from roots of local rice plant Kamba in Central Sulawesi, Indonesia. Biodiversitas 21: 1614-1624. The local Kamba rice plants are cultivated by the Bada Valley people for generations by obtaining seeds from previous crops. This rice plant is able to s...
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Context 1
... ability of endophytic bacteria isolates to dissolve phosphate was qualitatively determined by culturing bacterial isolates in Pikovskaya solid media modified with the addition of Bromophenol blue 0.01 g L -1 . The test results showed that 19 isolates of endophytic bacteria were shown to be able to form clear zones around bacterial colonies on solid Pikovskaya media (Figure 8) with different clear zone diameters and colony diameters (Table 2). Indicator of the ability of bacteria to dissolve phosphate is characterized by its ability to grow on solid Pikovskaya media and form clear zones around the colony. ...
Context 2
... best phosphate solvent bacteria's ability to produce the diameter of the halo zone with the largest area compared to other bacteria isolates colonies, while isolates that do not form clear zones are unable to dissolve phosphate. The results showed that all endophytic bacteria isolates were able to dissolve phosphates with different colony diameters and clear zone diameters produced ( Figure 8; Table 2). ...
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Citations
... This causes DNA to come out of the cell and form slimy threads when removed from the surface of the slide. In contrast, Gram-positive bacteria do not form threads when removed from the surface of the slide because of their thick Peptidoglycan layer, so these bacteria do not lyse easily when dropped with a 3% KOH solution (Sudewi et al., 2020). The catalase reaction test yielded positive catalase results of 83.33%, while catalase negatives were 16.66% ( Table 2). ...
... Hallmann et al. [57] stated that crop rotation factors and soil conditions influence the population structure of bacteria. Another factor according to Sudewi et al. [58] is the presence of phytopathogens from the location of endophytic bacteria collection. ...
Stem base rot (SBR) disease caused by Phytophthora capsici Leonian is associated with significant yield loss. Meanwhile, wild passion fruit (Passiflora foetida L.) interacts with bacteria that have potential to control SBR disease. Therefore, this research aimed to examine potential of endophytic bacteria from wild passion fruit to control SBR disease in pepper plant and the role as growth promoters. A total of 12 endophytic bacteria isolates were tested for potential to promote growth and biocontrol, focusing on the ability to produce the phytohormone IAA, dissolve phosphate, fix nitrogen, as well as produce cellulase and protease enzymes. The three best isolates were then tested on pepper cuttings (Var. Malonan I). The results showed that the highest disease severity was found in control at 40.74% (moderately susceptible), followed by isolate KSA 01 at 37.03%, and SIB 01 at 33.33% (resistant). Disease severity in isolate KPA 03 was significantly lower at 7.41% (very resistant). The highest disease incidence occurred in control and KSA 03 at 44.44%, while the lowest occurred in KPA 03 and SIB 02 at 33.33%. Isolate KPA 03 produced the highest plant height increase (39.87 cm) and the highest number of leaves (5.78), although it was not significantly different from isolate SIB 02. This research showed that endophytic bacteria from wild passion fruit could effectively control SBR disease and improve pepper plant growth.
... Endophytic bacteria isolated from the roots of local rice plant from Indonesia showed a very effective result in IAA production and phosphate solubilisation (18). ...
Endophytic bacteria are those which live inside the plant tissue without harming the plants and can be isolated from almost all parts of the plant like roots, leaves, stems, fruits, seeds, flowers etc. These microbes were found to help the host plant in disease management, environmental stress tolerance and also help the plant in their growth and development. Previousy, it was suggested that the use of endophytic bacteria as a biofertilizer can replace the use of several agrochemicals in agriculture. Endophytic bacteria like Herbaspirillum sp., Methylobacteriumsp., and Brevundimonassp. isolated from tea plant showed distinct plant growth promoting activities. The main aim of this chapter is to discuss about the endophytic bacteria isolated from different plants and their growth promotion activities. Additionally, it will be discussed about how endophytic bacteria help plants to cope with different environmental stresses and manage their diseases.
... Proses pelatihan dilakukan dengan mempraktekkan langsung cara membuat pupuk organik cair dengan bahan dasar limbah urin sapi serta bahan-bahan lain yang mudah diperoleh. Bahan yang digunakan berupa urin sapi 2 L, air kelapa 2 L, air cucian beras 1 liter, gula merah 0,5 kg yang diencerkan berperan sebagai sumber energi bagi pertumbuhan mikroorganisme (Pramana, Setiyo & Aviantara, 2018), serta starter pengganti EM4 yaitu BioDera M-6 sebanyak 250 mL yang merupakan bioaktivator hasil penelitian (Sudewi et al., 2020;Sudewi et al., 2021;Ratnawati et al., 2019). ...
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... Testing the morphological characteristics of the colony which included color, form, elevation, and edge were the characterization procedures [13]. The bacteria that were presumptive on B. glumae as described by Aflaha et al. [6], Coenye and Vandamme [14], and Singh and Vishunavat [15] were chosen to perform physiological characterization. ...
Bacterial Grain Rot caused by Burkholderia glumae is an important disease of rice. In South Sulawesi, this seed-borne pathogen has been detected in several places. There were some reports from the farmers regarding rice yield loss due to this disease. This study aimed to isolate and characterize morpho-physiologically bacterium causing grain rot of rice in the main rice-producing areas of Gowa Regency, South Sulawesi. Samples were obtained from 10 districts. Several grains of rice were isolated and grown in King’s B medium. Morphological characteristics were determined by the color, form, edge, and elevation of colonies. KOH solubility, catalase test, anaerobic growth, toxoflavin production assay, production of fluorescent pigment, and colony color on YDC medium were performed to determine physiological characteristics. 1 isolate from each area was found which has similar morphological traits to B. glumae . Colonies that were yellow, yellowish white, creamy white, and grayish white, with a circular form, entire edge, and convex elevation. The result of physiological characterization showed that all isolates tested positive for KOH solubility and catalase test as well. All isolates showed negative reactions for anaerobic growth, production of fluorescent pigment, and colony color on YDC medium. 5 isolates were able to produce yellow pigment (toxoflavin), so it can be concluded that all of the isolates that have been characterized morpho-physiologically are presumably confirmed as B. glumae .
... The structure of the cell wall was the cause of this variation. Grampositive bacteria have peptidoglycan-based cell walls, whereas, Gram-negative bacteria have lipid-based cell walls (Sudewi et al., 2020). ...
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... Gram-positive bacteria have thick and rigid cell walls because they are composed of peptidoglycan with more composition than Gram-negative bacteria have thin cell walls, so the Potassium Hydroxide used in the test will more easily break down the cell walls of Gram-negative bacteria. Sudewi et al. 2020). The catalase test on bacterial isolates found that 15 isolates reacted positively and one isolate reacted negatively. ...
Ratnawati, Sudewi S, Jaya K, Saleh AR. 2022. Microbial exploration from two different ecosystems in Central Sulawesi, Indonesia. Biodiversitas 23: 6089-6096. A supportive growing environment and the presence of microbes in the soil are considered good habitats for plant growth. Endophytic microbes live in symbiosis (mutual benefit) with their host plants, both in the stem and root tissues of plants, providing defense services in biotic and abiotic stress situations. This study was aimed to identify various microbes that exist in various agricultural cultivation ecosystems in Central Sulawesi. Microbial sampling was obtained from healthy plant tissues (endophytes) and rhizosphere in lowland rice and shallot plantation ecosystems. The sample obtained was then weighed as much as 5 g and surface sterilized for further isolation by serial dilution method to be spread on NA (Nutrient Agar) media for bacteria, and PDA (Potato Dextrose Agar) for fungi. The microbes that had grown were re-isolated to obtain pure isolate culture of microbes. The results showed that 8 isolates from the rhizosphere and 4 isolates as endophytes in the ecosystem of organic rice fields were recorded. The fungal isolates from the shallot plantation "Lembah Palu" found as many as 12 isolates from rhizosphere and 4 isolates as endophytes. The results of macroscopic and microscopic characteristics on fungal isolates identified as the species of Trichoderma, Fusarium, Penicillium, Gliocladium and Aspergillus.
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Abstrak Bakteri mampu menghasilkan siderofor umtuk mengkelat besi dalam lingkungan dan digunakan oleh tanaman sebagai kofaktor dalam pembentukan klorofil untuk pembentukan glukosa. Penelitian ini bertujuan untuk mendapatkan bakteri penghasil siderofor tinggi dari akar nanas Simadu, Kabupaten Subang Provinsi Jawa Barat. Bakteri diisolasi dari akar nanas Simadu, kemudian diseleksi dengan membedakan morfologinya dan diidentifikasi sebagai penghasil siderofor pada media Chrome Azurol S (CAS). Diperoleh 10 isolat bakteri (M1 sampai dengan M10) yang mampu menghasilkan siderofor. Isolat bakteri M7 memiliki kemampuan menghasilkan siderofor tertinggi. Isolat M7 teridentifikasi sebagai bakteri Gram negatif. Hasil analisis pohon filogenetik berdasarkan sekuensing 16S rDNA menunjukkan isolat kelompok genus Providencia. Dibandingkan dengan Providencia vermicola , isolat standar dari InaCC yang berasal dari akar Curcuma zedoaria, M7 menunjukkan produksi siderofor yang lebih tinggi pada media LB pada kondisi aerobik.[Kata kunci: Isolasi bakteri, Providencia, akumulasi siderofor, akar nanas Simadu Subang]Abstract Bacteria can produce siderophores for chelating iron in environments and are used by plants as an ingredient cofactor in building chlorophyll for glucose production. This study aims to obtain high siderophore-producing bacteria from the roots of pineapple Simadu, Subang District, West Java Province. Bacteria were isolated from Simadu pineapple roots, then selected by distinguishing their morphology and identified as producing siderophores with Chrome Azurol S (CAS) media. Obtained 10 bacterial isolates (M1 to M10) capable of producing siderophores. Bacterial isolate M7 had the highest siderophore production ability. M7 isolate was identified as Gram-negative bacteria. The results of the phylogenetic tree analysis based on 16S rDNA sequencing showed this isolate as the genus Providencia. Compared to the Providencia vermicola as reference isolate from InaCC derived from the roots of Curcuma zedoaria, M7 showed higher siderophore production in LB media under aerobic conditions.[Keywords: Bacteria isolation, Providencia, siderophore accumulation, Simadu Subang pineapple root]
