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The qualitative analysis of endophytic bacteria isolates to solubilize phosphate on Pikovskaya medium with the addition of Bromophenol blue 0.01 g L -1
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Sudewi S, Ala A, Baharuddin, Farid M. 2020. The isolation, characterization endophytic bacteria on from roots of local rice plant Kamba in Central Sulawesi, Indonesia. Biodiversitas 21: 1614-1624. The local Kamba rice plants are cultivated by the Bada Valley people for generations by obtaining seeds from previous crops. This rice plant is able to s...
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Context 1
... ability of endophytic bacteria isolates to dissolve phosphate was qualitatively determined by culturing bacterial isolates in Pikovskaya solid media modified with the addition of Bromophenol blue 0.01 g L -1 . The test results showed that 19 isolates of endophytic bacteria were shown to be able to form clear zones around bacterial colonies on solid Pikovskaya media (Figure 8) with different clear zone diameters and colony diameters (Table 2). Indicator of the ability of bacteria to dissolve phosphate is characterized by its ability to grow on solid Pikovskaya media and form clear zones around the colony. ...
Context 2
... best phosphate solvent bacteria's ability to produce the diameter of the halo zone with the largest area compared to other bacteria isolates colonies, while isolates that do not form clear zones are unable to dissolve phosphate. The results showed that all endophytic bacteria isolates were able to dissolve phosphates with different colony diameters and clear zone diameters produced ( Figure 8; Table 2). ...
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Endophytic bacteria are microorganisms that thrive in plant tissue without causing any negative effects to the host plants. The purpose of this study was to apply endophytic bacteria that have the potential to produce IAA hormones as biological agents in rice seeds. This research was conducted by testing IAA hormone using a spectrophotometer, testi...
Citations
... Hallmann et al. [57] stated that crop rotation factors and soil conditions influence the population structure of bacteria. Another factor according to Sudewi et al. [58] is the presence of phytopathogens from the location of endophytic bacteria collection. ...
Stem base rot (SBR) disease caused by Phytophthora capsici Leonian is associated with significant yield loss. Meanwhile, wild passion fruit (Passiflora foetida L.) interacts with bacteria that have potential to control SBR disease. Therefore, this research aimed to examine potential of endophytic bacteria from wild passion fruit to control SBR disease in pepper plant and the role as growth promoters. A total of 12 endophytic bacteria isolates were tested for potential to promote growth and biocontrol, focusing on the ability to produce the phytohormone IAA, dissolve phosphate, fix nitrogen, as well as produce cellulase and protease enzymes. The three best isolates were then tested on pepper cuttings (Var. Malonan I). The results showed that the highest disease severity was found in control at 40.74% (moderately susceptible), followed by isolate KSA 01 at 37.03%, and SIB 01 at 33.33% (resistant). Disease severity in isolate KPA 03 was significantly lower at 7.41% (very resistant). The highest disease incidence occurred in control and KSA 03 at 44.44%, while the lowest occurred in KPA 03 and SIB 02 at 33.33%. Isolate KPA 03 produced the highest plant height increase (39.87 cm) and the highest number of leaves (5.78), although it was not significantly different from isolate SIB 02. This research showed that endophytic bacteria from wild passion fruit could effectively control SBR disease and improve pepper plant growth.
... Endophytic bacteria isolated from the roots of local rice plant from Indonesia showed a very effective result in IAA production and phosphate solubilisation (18). ...
Endophytic bacteria are those which live inside the plant tissue without harming the plants and can be isolated from almost all parts of the plant like roots, leaves, stems, fruits, seeds, flowers etc. These microbes were found to help the host plant in disease management, environmental stress tolerance and also help the plant in their growth and development. Previousy, it was suggested that the use of endophytic bacteria as a biofertilizer can replace the use of several agrochemicals in agriculture. Endophytic bacteria like Herbaspirillum sp., Methylobacteriumsp., and Brevundimonassp. isolated from tea plant showed distinct plant growth promoting activities. The main aim of this chapter is to discuss about the endophytic bacteria isolated from different plants and their growth promotion activities. Additionally, it will be discussed about how endophytic bacteria help plants to cope with different environmental stresses and manage their diseases.
... Testing the morphological characteristics of the colony which included color, form, elevation, and edge were the characterization procedures [13]. The bacteria that were presumptive on B. glumae as described by Aflaha et al. [6], Coenye and Vandamme [14], and Singh and Vishunavat [15] were chosen to perform physiological characterization. ...
Bacterial Grain Rot caused by Burkholderia glumae is an important disease of rice. In South Sulawesi, this seed-borne pathogen has been detected in several places. There were some reports from the farmers regarding rice yield loss due to this disease. This study aimed to isolate and characterize morpho-physiologically bacterium causing grain rot of rice in the main rice-producing areas of Gowa Regency, South Sulawesi. Samples were obtained from 10 districts. Several grains of rice were isolated and grown in King’s B medium. Morphological characteristics were determined by the color, form, edge, and elevation of colonies. KOH solubility, catalase test, anaerobic growth, toxoflavin production assay, production of fluorescent pigment, and colony color on YDC medium were performed to determine physiological characteristics. 1 isolate from each area was found which has similar morphological traits to B. glumae . Colonies that were yellow, yellowish white, creamy white, and grayish white, with a circular form, entire edge, and convex elevation. The result of physiological characterization showed that all isolates tested positive for KOH solubility and catalase test as well. All isolates showed negative reactions for anaerobic growth, production of fluorescent pigment, and colony color on YDC medium. 5 isolates were able to produce yellow pigment (toxoflavin), so it can be concluded that all of the isolates that have been characterized morpho-physiologically are presumably confirmed as B. glumae .
... The structure of the cell wall was the cause of this variation. Grampositive bacteria have peptidoglycan-based cell walls, whereas, Gram-negative bacteria have lipid-based cell walls (Sudewi et al., 2020). ...
Phosphate-solubilizing and nitrogen-fixing bacteria are crucial in increasing soil fertility and restoring soil properties damaged by salinity and other abiotic environmental factors. The presented study aims to explore and identify the morphological characteristics of phosphate-solubilizing and nitrogen-fixing bacteria in saline soil. This study took place from August until November 2022 in the field and laboratory of the Faculty of Agriculture, Universitas Muhammadiyah Sumatera Utara, Medan, Indonesia. Isolation of potential microbes proceeded to characterize phosphate-solubilizing and nitrogen-fixing bacteria. Sampling began with the soil planted with rice and palm oil at the sampling location. Morphological parameters observed were color, form, margin, surface, and elevation of pure colonies. The result showed that exploring various species in saline soil revealed 19 colonies and 14 cells of phosphate-solubilizing bacteria and 16 colonies and 14 cells of nitrogen-fixing bacteria. The pure colonies of the phosphate-solubilizing and nitrogen-fixing bacteria showed differences in the morphological characteristics, i.e., color, form, margin, surface, and elevation. The potential microbes obtained sought to increase soil fertility and crop production.
... The physiological characterization of bacteria was carried out by several tests, including: the gram test using 3% KOH solution and bacterial gram staining (Mu'minah et al. 2015;Begum et al. 2017); the oxidase test used Sigma strip oxidase contains plastic strips with a paper zone saturated with a solution of N,N-dimethyl-1,4phenylene diamine and alpha-naphthol, catalase test (Sudewi et al. 2020); oxidation fermentation test, tolerance test for temperature conditions of 37-40°C and acid using 5% NaCl, fried egg colony test on PDA media (Thakkar et al. 2016); sensitivity test to 15 µg erythromycin antibiotic (Kanzil et al. 2015), and fluorescent pigment production using King's B media (Nepali et al. 2018). In addition, enzyme activity tests were conducted on the ability of bacteria to produce enzymes: protease, lecithinase, and pectolytic enzymes, following the methods of Boluk et al. (2021), Thakur et al. (2021) and Kumvinit and Akarapisan (2019). ...
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... Some research has demonstrated that PGPB can be effective in increasing the production of phytohormones (e.g., auxins, cytokinins, and gibberellins) (Sudewi et al. 2020). The production of some phytohormones, such as ABA, by PGPB can be due to aquaporin upregulation under stress conditions (Krishnamoorthy et al. 2022). ...
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... Gram-positive bacteria have thick and rigid cell walls because they are composed of peptidoglycan with more composition than Gram-negative bacteria have thin cell walls, so the Potassium Hydroxide used in the test will more easily break down the cell walls of Gram-negative bacteria. Sudewi et al. 2020). The catalase test on bacterial isolates found that 15 isolates reacted positively and one isolate reacted negatively. ...
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Abstrak Bakteri mampu menghasilkan siderofor umtuk mengkelat besi dalam lingkungan dan digunakan oleh tanaman sebagai kofaktor dalam pembentukan klorofil untuk pembentukan glukosa. Penelitian ini bertujuan untuk mendapatkan bakteri penghasil siderofor tinggi dari akar nanas Simadu, Kabupaten Subang Provinsi Jawa Barat. Bakteri diisolasi dari akar nanas Simadu, kemudian diseleksi dengan membedakan morfologinya dan diidentifikasi sebagai penghasil siderofor pada media Chrome Azurol S (CAS). Diperoleh 10 isolat bakteri (M1 sampai dengan M10) yang mampu menghasilkan siderofor. Isolat bakteri M7 memiliki kemampuan menghasilkan siderofor tertinggi. Isolat M7 teridentifikasi sebagai bakteri Gram negatif. Hasil analisis pohon filogenetik berdasarkan sekuensing 16S rDNA menunjukkan isolat kelompok genus Providencia. Dibandingkan dengan Providencia vermicola , isolat standar dari InaCC yang berasal dari akar Curcuma zedoaria, M7 menunjukkan produksi siderofor yang lebih tinggi pada media LB pada kondisi aerobik.[Kata kunci: Isolasi bakteri, Providencia, akumulasi siderofor, akar nanas Simadu Subang]Abstract Bacteria can produce siderophores for chelating iron in environments and are used by plants as an ingredient cofactor in building chlorophyll for glucose production. This study aims to obtain high siderophore-producing bacteria from the roots of pineapple Simadu, Subang District, West Java Province. Bacteria were isolated from Simadu pineapple roots, then selected by distinguishing their morphology and identified as producing siderophores with Chrome Azurol S (CAS) media. Obtained 10 bacterial isolates (M1 to M10) capable of producing siderophores. Bacterial isolate M7 had the highest siderophore production ability. M7 isolate was identified as Gram-negative bacteria. The results of the phylogenetic tree analysis based on 16S rDNA sequencing showed this isolate as the genus Providencia. Compared to the Providencia vermicola as reference isolate from InaCC derived from the roots of Curcuma zedoaria, M7 showed higher siderophore production in LB media under aerobic conditions.[Keywords: Bacteria isolation, Providencia, siderophore accumulation, Simadu Subang pineapple root]
... Isolat bakteri endofit diperoleh dari rizosfer padi lokal Kamba Lembah Bada, dikulturkan pada media NA (Nutrient Agar) padat dan diinkubasi selama 48 jam pada suhu 28 0 C. Koloni bakteri yang tumbuh selanjutnya disuspensikan dalam aquadest steril hingga mencapai kerapatan populasi mencapai 10 9 CFU mL -1 (Sudewi et al., 2020). Adapun bakteri endofit yang digunakan dalam pengujian ini terdiri dari 4 jenis isolat yaitu RKGU4, RKGU7, RKGU8 dan RKGU15. ...
Bakteri endofit memiliki kemampuan untuk hidup dan menginvasi jaringan tanaman serta mempunyai sifat antagonis terhadap patogen tanaman dengan berbagai mekanisme. Penelitian bertujuan untuk mengetahui mekanisme dan persentase daya penghambatan oleh isolat bakteri endofit asal padi lokal Kamba secara in vitro dalam mengendalikan penyakit bercak ungu pada tanaman bawang merah. Pelaksanaan penelitian bertempat di Laboratorium Mikrobiologi Fakultas Pertanian Universitas Alkhairaat Palu. Metode penelitian secara kualitatif dengan melakukan uji reaksi hipersensitivitas pada daun tembakau dan pengujian kemampuan isolat bakteri endofit dalam menghasilkan senyawa HCN. Secara kuantitatif dengan mengamati daya hambat dari isolat bakteri endofit terhadap cendawan pathogen uji. Penelitian menggunakan rancangan acak lengkap yang terdiri atas 4 perlakuan isolat bakteri endofit (RKGU4, RKGU7, RKGU8 dan RKGU15) dengan 3 ulangan. Dari hasil penelitian diperoleh 2 isolat bakteri endofit yang memiliki kemampuan terbaik dalam menghambat pertumbuhan koloni cendawan Alternaria porri yakni RKGU8 dan RKGU15. Selain itu kedua isolat tidak berpotensi sebagai patogen berdasarkan uji reaksi hipersensitivitas serta memiliki kemampuan dalam memproduksi senyawa HCN. Isolat bakteri endofit memiliki potensi untuk dikembangkan sebagai agens pengendali hayati yang ramah lingkungan.
... Some research has demonstrated that PGPB can be effective in increasing the production of phytohormones (e.g., auxins, cytokinins, and gibberellins) [36]. The production of some phytohormones, such as ABA, by PGPB can be due to aquaporin upregulation under stress conditions [37]. ...
This is preprint but it's published now in Biotechnology report that there's in below link:
http://dx.doi.org/10.1016/j.btre.2023.e00781
