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The influence of the R. obtusifolius extract alone and in combination with 5-FU on TNFa (A), COX-2 (B), VEGFa (C), and MMP-2 (D) in A549 cells. Control—A549C, 5-Fluorouracil—5-FU (40 μM), Rumex obtusifolius—RO (0.25 mg/mL), ROFU—RO + 5-FU (0.25 mg/mL + 40 μM). Each test sample was added to three different passages in triplicate (n = 3, *—p ≤ 0.05, **—p ≤ 0.01, ns—non-significant).

The influence of the R. obtusifolius extract alone and in combination with 5-FU on TNFa (A), COX-2 (B), VEGFa (C), and MMP-2 (D) in A549 cells. Control—A549C, 5-Fluorouracil—5-FU (40 μM), Rumex obtusifolius—RO (0.25 mg/mL), ROFU—RO + 5-FU (0.25 mg/mL + 40 μM). Each test sample was added to three different passages in triplicate (n = 3, *—p ≤ 0.05, **—p ≤ 0.01, ns—non-significant).

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... Total and phospho-PI 3 kinase p85 were each assayed in triplicate using the phospho-and total PI 3 Kinase p85 antibodies included in the PI 3 Kinase Kit. Phospho-PI 3 kinase p85 and Total PI3K levels were measured using an In-Cell ELISA kit (ab207484) according to the manufacturer's instructions [28]. ...
... The assay was performed in A549 cell culture supernatant using the MMP-2 ELISA kit according to the manufacturer's instructions (protocol number ab267646) [28]. Test samples included the control group (distilled water) and GSAg Np (5.75 μg/mL). ...
... Following the final wash, 100 μL of TMB One-Step Substrate Reagent was added to each well and incubated for 30 min. In the last stage of the experiment, 50 μL of Stop Solution was added to each well, and the absorbance was measured at 450 nm using the manufacturer's instructions (ab267646) [28]. ...
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