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The frequency of hybridization signals on chromosomes of Vicia species with Bam HI family repetitive sequence probes.
Source publication
The organisation of DNA sequences at chromosomal level in twelve Vicia species, more particularly among V. faba and its close wild relatives (V. narbonensis, V. hyaeniscyamus, V. johannis, V. galilaea, V. bithynica), has been performed using fluorescence in situ hybridization (FISH). The four BamHI discrete size classes of 850, 900, 990 and 1750-bp...
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Citations
... It helps to clarify genetic maps and to assign linkage groups to physically marked chromosomes as well [15][16][17][18]. Florescence in situ hybridization has been applied in different Vicia species [19][20][21][22][23][24][25]. ...
... Molecular cytogenetic approaches like florescence in situ hybridization (FISH) have been applied in different Vicia species [23,24,[49][50][51]. Faba bean (Vicia faba) is the most extensively studied species via in situ hybridization tool to localize the distribution of repetitive sequences and 5S and 45S rDNA on its chromosomes [25,[52][53][54][55][56][57]24]. ...
... Molecular cytogenetic approaches like florescence in situ hybridization (FISH) have been applied in different Vicia species [23,24,[49][50][51]. Faba bean (Vicia faba) is the most extensively studied species via in situ hybridization tool to localize the distribution of repetitive sequences and 5S and 45S rDNA on its chromosomes [25,[52][53][54][55][56][57]24]. Ali and Osman Journal of Genetic Engineering and Biotechnology (2020) 18:37 studied FISH with multiple repeated DNA (four BamHI classes) isolated from V. faba and probes of 5S rDNA and 25S rDNA. ...
Background: Genus Vicia is a member of family Fabaceae and comprises 180 to 210 species. The most important species is faba bean (Vicia faba) which is still one of the most favourable grain legumes over all the world. The genus contains some additional food crops and a number of forage plants and some other weedy strains such as Vicia angustifolia and Vicia cordata. The aim of the present investigation is to elucidate the phylogenetic relationships among four Vicia species, two species (Vicia angustifolia L. ssp. Angustifolia (2n = 12) and Vicia cordata wulfen ex Hoppe (2n = 10)) belong to section Vicia, Vicia dalmatica A. Kern (2n = 12, section Cracca), and Vicia johannis tamamsch (2n = 14, section Faba).
... It helps to clarify genetic maps and to assign linkage groups to physically marked chromosomes as well [15][16][17][18]. Florescence in situ hybridization has been applied in different Vicia species [19][20][21][22][23][24][25]. ...
... Molecular cytogenetic approaches like florescence in situ hybridization (FISH) have been applied in different Vicia species [23,24,[49][50][51]. Faba bean (Vicia faba) is the most extensively studied species via in situ hybridization tool to localize the distribution of repetitive sequences and 5S and 45S rDNA on its chromosomes [25,[52][53][54][55][56][57]24]. ...
... Molecular cytogenetic approaches like florescence in situ hybridization (FISH) have been applied in different Vicia species [23,24,[49][50][51]. Faba bean (Vicia faba) is the most extensively studied species via in situ hybridization tool to localize the distribution of repetitive sequences and 5S and 45S rDNA on its chromosomes [25,[52][53][54][55][56][57]24]. Ali and Osman Journal of Genetic Engineering and Biotechnology (2020) 18:37 studied FISH with multiple repeated DNA (four BamHI classes) isolated from V. faba and probes of 5S rDNA and 25S rDNA. ...
Background:
Genus Vicia is a member of family Fabaceae and comprises 180 to 210 species. The most important species is faba bean (Vicia faba) which is still one of the most favourable grain legumes over all the world. The genus contains some additional food crops and a number of forage plants and some other weedy strains such as Vicia angustifolia and Vicia cordata. The aim of the present investigation is to elucidate the phylogenetic relationships among four Vicia species, two species (Vicia angustifolia L. ssp. Angustifolia (2n = 12) and Vicia cordata wulfen ex Hoppe (2n = 10)) belong to section Vicia, Vicia dalmatica A. Kern (2n = 12, section Cracca), and Vicia johannis tamamsch (2n = 14, section Faba).
Results:
Two tools have been applied to identify the genetic relationships among the examined species, double fluorescence in situ hybridization (FISH) has been used to localize the sites of 5S and 45S rDNA, and sodium dodecyl sulfate-poly acrylamide gel electrophoretic (SDS-PAGE) patterns of total seed storage protein fractions. Double FISH experiment has not shown any variation in the loci number, but the positions along the chromosomes were different; both Vicia johannis and Vicia dalmatica exhibited the same interstitial 45S rRNA gene loci, while Vicia angustifolia and Vicia cordata have shown single large stretched 45S rRNA loci almost at the terminal region of the shortest chromosome. It could be concluded from the similarity matrix among the Vicia species as computed according to Jaccard coefficient from the SDS-PAGE, that V. cordata is similar to V. angustifolia and V. dalmatica by a percentage of 73 and 69%, respectively, and the most related species to V. johannis is V. dalmatica (~ 64%).
Conclusion:
FISH and SDS-PAGE of the total seed storage proteins together reflected the similar genetic relationship among the studied species as fellows, V. angustifolia is more related to V. cordata then comes V. dalmatica and then V. johannis which is at a distal position from the other species.
... Thus, there was no need for testing incubations other than these described in the BMaterials and methods^section. The detected arrangement of rDNA loci in A. cepa (Fig. 1b), N. damascena (Fig. 1c), and V. faba (Fig. 1d-e) conformed to the rDNA chromosomal pattern already described by other authors (Hizume 1992(Hizume , 1993Shibata and Hizume 2002;Raina et al. 2010;Hizume et al. 2013). The novel findings include (i) the 18S-5.8S-26S ...
The major drawbacks of standard plant fluorescence in situ hybridization (FISH) designed for double-stranded DNA probes include requirement for experimentally determined heat denaturation of chromosomes at high temperatures and at least overnight hybridization. Consequently, processing with chromosomal preparations may easily result in heat-induced deterioration of chromosomal structural details, is time-consuming, and involves the use of toxic formamide and formaldehyde. Here, I have described a simple and appealing non-toxic procedure with ethylene carbonate (EC)—a formamide-substituting solvent and double-stranded repetitive DNA probes. Applying EC as a component of the hybridization solution at 46 °C not only allowed successful overnight hybridization but also gave a possibility to reduce the hybridization time to 3 h, hence converting the technique into a 1-day procedure. Importantly, the EC-FISH tended to preserve well chromosome structural details, e.g., DAPI-positive bands, thus facilitating simultaneous FISH mapping and chromosome banding on the same slide. The procedure requires no formaldehyde and RNA-se treatment of chromosomes, and no heat denaturation of chromosomal DNA. The key condition is to obtain high-quality cytoplasm-free preparations. The method was reproducible in all the plants studied (Allium, Nigella, Tradescantia, Vicia), giving a species-specific signal pattern together with clear DAPI bands on chromosomes. The procedure described here is expected to give a positive stimulus for improving gene-mapping approaches in plants.
Electronic supplementary material
The online version of this article (10.1007/s00709-019-01345-7) contains supplementary material, which is available to authorized users.
... Besides conflicting accounts with regard to the circumscription and delineation of the species as above, the nomenclature and taxonomic rank below species level of many of the taxa remains highly controversial. For example, the nomenclature, taxonomic rank and phylogenetic relationships of the taxa within the three species complexes, Narbonensis, Sativa and Villosa, have been inconsistent and debatable (Venora et al. 2000;Fennel et al. 1998;Raina and Ogihara 1995;Raina et al. 2001, Shiran andRaina 1990, Raina and Bisht 1988, Raina et al. 2010Bennett and Maxted 1997). Based on the morphological characters and karyotypes, several species have been moved in and out of the sections (Kupicha 1976;Hanelt and Mettin 1989;Leht 2009;Maxted and Douglas 1997;Ruffini Castiglione et al. 2007. ...
... Based on the morphological characters and karyotypes, several species have been moved in and out of the sections (Kupicha 1976;Hanelt and Mettin 1989;Leht 2009;Maxted and Douglas 1997;Ruffini Castiglione et al. 2007. There are also conflicting arguments with regard to the origin of widely cultivated species, Vicia faba (Perrino and Pignone 1981;Zohary and Hopf 1973;Schäfer 1973;Raina 1990;Raina and Rees 1983;Raina andOgihara 1994, 1995;Venora et al. 2000;Van de Ven et al. 1993;Jaaska 1997;Potokina et al. 1999;Leht and Jaaska 2002;Frediani et al. 2005;Raina et al. 2010;Koul et al. 1999). ...
... The characterization of genomes by DNA markers and linear differentiation of chromosomes have recently been increasingly utilized in Vicia for taxonomic and phylogenetic studies (Shiran and Raina 2001;Raina et al. 2001Raina et al. , 2010Raina and Narayan 1984;Raina andOgihara 1994, 1995;Raina and Rees 1983;Ruffini Castiglione et al. 2007. Most of these studies have succeeded in resolving a few taxonomic riddles, but much remains to be desired. ...
The ITS1-5.8S-ITS2 region of the 18S-28S nrDNA was sequenced in 49 Vicia species and subspecies selected from nine sections of the two subgenera to resolve taxonomic contradictions. Phylogenies derived from the internal transcribed spacer (ITS) sequences strongly support the view that both Vicia faba and Vicia bithynica are distant from the Narbonensis species complex (NSC) in section Faba. V. faba is more closely aligned with sections Peregrinae and Hypechusa, whereas V. bithynica is more closely aligned with section Vicia. Six synapomorphic substitutions unique to the NSC support a separate section for the Narbonensis complex. Vicia johannis subsp. johannis was the most diverged species within the NSC with high genetic distance between V. johannis subsp. johannis and remaining species and subspecies of the NSC. These results suggest that Vicia johannis subsp. johannis should be given species rank. The neighbor-joining and parsimony based trees supported the sections Vicia (after the exclusion of Vicia lathyroides) and Cracca (after the exclusion of Vicia vicioides and Vicia hirsuta). The section Hypechusa is polyphyletic and paraphyletic based on the present ITS sequence cladistic analysis. The x = 7 as the ancestral number for the genus is supported by the present study.
The composition of nuclear DNA and mitotic time cycle in Vicia faba, commonly known as Faba bean, and its close relatives grouped together in Narbonensis species complex are compared. The melting profile and buoyant density gradient analysis in the species, V. narbonensis, V. serratifolia, V. galilaea and V. hyaeniscyamus belonging to Narbonensis species complex and V. faba did not show any appreciable change in the base composition. The density gradient profiles in the species belonging to the complex showed the presence of a distinct satellite fraction in each species. This satellite fraction was absent in V. faba. The change in the total duration or the duration of different stages of mitotic cycle in the species belonging to the species complex was not significantly different. In comparison, V. faba had longer mitotic cycle duration.
In this study, 11 Vicia taxa naturally distributed in Turkey were karyologically studied. Chromosome numbers, karyotypes and idiograms of five taxa that belong to section Cracca of the genus Vicia (Vicia cracca subsp. gerardii, V. cracca subsp. atroviolacea, V. cracca subsp. stenophylla, V. canescens subsp. canescens, V. palaestina) and six taxa that belong to section Vicia (Vicia michauxii var. stenophylla, V. pannonica var. pannonica, V. hybrida, Vicia sativa subsp. nigra var. nigra, Vicia sativa subsp. nigra var. segetalis, Vicia sativa subsp. incisa var. cordata) were done. The chromosome numbers of these taxa were found as 2n = 10, 12, 14, 24. The chromosome numbers and karyotype analysis of five of these were reported for the first time and chromosome morphology of two taxa was identified for the first time. Also chromosome numbers of six taxa were verified. These results will contribute to the taxonomy of the genus Vicia which has a high diversity in Turkey.
