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The development of T. belorussicum explants (a, e, i, m, q, u cotyledon; b, f, j, n, r, v meristem; c, g, k, o, s, w hypocotyl; d, h, l, p, t root) cultured on: P1-½ MS (control), P2-½ MS + PSK,

The development of T. belorussicum explants (a, e, i, m, q, u cotyledon; b, f, j, n, r, v meristem; c, g, k, o, s, w hypocotyl; d, h, l, p, t root) cultured on: P1-½ MS (control), P2-½ MS + PSK,

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Taraxacum belorussicum Val. N. Tikhom, a poorly known and obligatory apomictic species, is an attractive plant material for studying the embryological, genetic and molecular mechanisms of apomixis. This work aims to obtain an efficient protocol for Taraxacum belorussicum regeneration. Four types of explants (cotyledons, hypocotyls, meristems and ro...

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... The samples were transferred to pure Technovit solution for 12 h and after polymerization of the resin, they were sectioned into 7 µm on a rotary microtome (Microm, Adams Instrumenten). The sections were stained with 0.1% toluidine blue O, naphthol blue black for proteins detection (Gałuszka et al., 2019), periodic acid-Schiff's (PAS) reagent for water-insoluble polysaccharides identification (Jensen, 1962), aniline blue for callose (Musiał et al., 2015), and mounted in Enthellan (Merck). ...
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