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Figure 3 - Protein Z Exerts Pro-Angiogenic Effects and Upregulates CXCR4

Figure 3.  Targeting PZ and CXCR4 with siRNA results in decreased migratory capacity and endothelial tube formation in vitro.
PZ and CXCR4 knock-down in unstimulated HUVECs as well as CXCR4 knock-down in PZ and SDF-1 stimulated HUVECs resulted in migration capacity comparable to that of ctrl after both 8 (A) and 24 hours (B). Data are given as box plots indicating the median with the 25th and 75th percentiles. Ø corresponds to unstimulated cells. ANOVA on ranks; $ p<0.05 vs. ctrl; n = 4 independent experiments. Representative images (C) and quantitative analysis (D) of capillary-like tube formation. HUVECs targeted with siRNA against PZ or CXCR4 showed significantly reduced capacity for capillary-like tube formation compared to ctrl. Addition of PZ or SDF-1 to CXCR4 siRNA targeted cells failed to restore endothelial tube formation. Data are given as box plots indicating the median with the 25th and 75th percentiles. Ø corresponds to unstimulated cells. ANOVA on ranks; $ p<0.05 vs. ctrl; n = 4 independent experiments.
Targeting PZ and CXCR4 with siRNA results in decreased migratory capacity and endothelial tube formation in vitro. PZ and CXCR4 knock-down in unstimulated HUVECs as well as CXCR4 knock-down in PZ and SDF-1 stimulated HUVECs resulted in migration capacity comparable to that of ctrl after both 8 (A) and 24 hours (B). Data are given as box plots indicating the median with the 25th and 75th percentiles. Ø corresponds to unstimulated cells. ANOVA on ranks; $ p<0.05 vs. ctrl; n = 4 independent experiments. Representative images (C) and quantitative analysis (D) of capillary-like tube formation. HUVECs targeted with siRNA against PZ or CXCR4 showed significantly reduced capacity for capillary-like tube formation compared to ctrl. Addition of PZ or SDF-1 to CXCR4 siRNA targeted cells failed to restore endothelial tube formation. Data are given as box plots indicating the median with the 25th and 75th percentiles. Ø corresponds to unstimulated cells. ANOVA on ranks; $ p<0.05 vs. ctrl; n = 4 independent experiments.
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