Sudlow site I (A) and site III (B) of human serum albumin (HSA) with bound site-specific ligands and potential catalytic amino acids. RWF, warfarin; BLA, 4Z,15E-bilirubin-IXalpha.

Sudlow site I (A) and site III (B) of human serum albumin (HSA) with bound site-specific ligands and potential catalytic amino acids. RWF, warfarin; BLA, 4Z,15E-bilirubin-IXalpha.

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Serum albumin possesses esterase and pseudo-esterase activities towards a number of endogenous and exogenous substrates, but the mechanism of interaction of various esters and other compounds with albumin is still unclear. In the present study, proton nuclear magnetic resonance (1H NMR) has been applied to the study of true esterase activity of alb...

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Context 1
... all lysines are excluded, while serines, threonines and tyrosines remain. To find possible catalytic amino acids in Sudlow site I and site III, we have analysed crystal structures of complexes of HSA with warfarin in Sudlow site I (PDB structure code 2bxd [23]) and HSA with 4Z,15E-bilirubin-IXalpha site III (PDB structure code 2vue [24]) (Figure 4). Despite the fact that our NMR experiments According to the data obtained, the accumulation of acetate in the presence of BSA is much more intense as compared with the spontaneous hydrolysis of NPA. ...
Context 2
... all lysines are excluded, while serines, threonines and tyrosines remain. To find possible catalytic amino acids in Sudlow site I and site III, we have analysed crystal structures of complexes of HSA with warfarin in Sudlow site I (PDB structure code 2bxd [23]) and HSA with 4Z,15E-bilirubin-IXalpha site III (PDB structure code 2vue [24]) (Figure 4). Despite the fact that our NMR experiments are performed in BSA, we chose HSA crystal structures for analysis since warfarin and 4Z,15E-bilirubin-IXalpha are site-specific ligands. ...
Context 3
... proposed that true esterase reaction occurs more likely in drug binding sites (Sudlow site I and site III) than in FA1-7, so we have not analysed albumin crystal structures encapsulating fatty acids. Figure 4 shows all amino acids within 6 Å of warfarin ( Figure 4A) and 4Z,15E-bilirubin-IXalpha ( Figure 4B) sorbed in Sudlow site I and site III, respectively. Analysis of this environment has revealed Tyr150 and Ser287 in Sudlow site I, as well as Tyr138, Tyr161, and Ser193 in site III. ...
Context 4
... proposed that true esterase reaction occurs more likely in drug binding sites (Sudlow site I and site III) than in FA1-7, so we have not analysed albumin crystal structures encapsulating fatty acids. Figure 4 shows all amino acids within 6 Å of warfarin ( Figure 4A) and 4Z,15E-bilirubin-IXalpha ( Figure 4B) sorbed in Sudlow site I and site III, respectively. Analysis of this environment has revealed Tyr150 and Ser287 in Sudlow site I, as well as Tyr138, Tyr161, and Ser193 in site III. ...
Context 5
... proposed that true esterase reaction occurs more likely in drug binding sites (Sudlow site I and site III) than in FA1-7, so we have not analysed albumin crystal structures encapsulating fatty acids. Figure 4 shows all amino acids within 6 Å of warfarin ( Figure 4A) and 4Z,15E-bilirubin-IXalpha ( Figure 4B) sorbed in Sudlow site I and site III, respectively. Analysis of this environment has revealed Tyr150 and Ser287 in Sudlow site I, as well as Tyr138, Tyr161, and Ser193 in site III. ...
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... three residues are the main candidates to be the catalytic amino acids responsible for true esterase activity of albumin. Figure 4. Sudlow site I (A) and site III (B) of human serum albumin (HSA) with bound site-specific ligands and potential catalytic amino acids. ...
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... all four studied binding sites, such a conformation of NPA has been revealed, in which NPA is in the immediate vicinity of the catalytic amino acid ( Figure 5). Figure 4 shows all amino acids within 6 Å of warfarin ( Figure 4A) and 4Z,15E-bilirubinIXalpha ( Figure 4B) sorbed in Sudlow site I and site III, respectively. Analysis of this environment has revealed Tyr150 and Ser287 in Sudlow site I, as well as Tyr138, Tyr161, and Ser193 in site III. ...
Context 8
... all four studied binding sites, such a conformation of NPA has been revealed, in which NPA is in the immediate vicinity of the catalytic amino acid ( Figure 5). Figure 4 shows all amino acids within 6 Å of warfarin ( Figure 4A) and 4Z,15E-bilirubinIXalpha ( Figure 4B) sorbed in Sudlow site I and site III, respectively. Analysis of this environment has revealed Tyr150 and Ser287 in Sudlow site I, as well as Tyr138, Tyr161, and Ser193 in site III. ...
Context 9
... all four studied binding sites, such a conformation of NPA has been revealed, in which NPA is in the immediate vicinity of the catalytic amino acid ( Figure 5). Figure 4 shows all amino acids within 6 Å of warfarin ( Figure 4A) and 4Z,15E-bilirubinIXalpha ( Figure 4B) sorbed in Sudlow site I and site III, respectively. Analysis of this environment has revealed Tyr150 and Ser287 in Sudlow site I, as well as Tyr138, Tyr161, and Ser193 in site III. ...

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