Figure 3 - available via license: Creative Commons Attribution 4.0 International
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Strict consensus tree of most parsimonious tree resulting from phylogenetic analysis of the combined sequences with heuristic search using maximum parsimony analysis. (Tree length of 578 steps, CI = 0.882, RI = 0.811, RC = 0.716 and HI =0.118). Numbers on the branches indicate bootstrap support and numbers below branches are Bayesian posterior probability values and clades are identified by letters.
Source publication
The phylogeny of the species of Potentilla in Kurdistan Region-Iraq was investigated by using six in-group species and one out-group related genus Sibbaldia parviflora, based on trnL-F intergenic region of chloroplast DNA and internal transcribed spacer of nuclear ribosomal DNA. In addition, the pollen morphology of the six species of Potentilla wa...
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Context 1
... exine sculpturing type includes striate pattern and consists of shallow, fingerprint-like ridges and predominately parallel arranged. (Plates 3-4). ...
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Pollen grains of ten wild and cultivar species were processed and examined microscopically with light and scanning electron microscopes. The results showed that all studied grains by LM had one pore (monoporate) and were very close in shape, their shapes ranged from spheroidal, sub spheroidal to oblate spheroidal in equatorial and polar view except...
Citations
... This was followed by 35 cycles of heating the sample to 94 degrees Celsius for 30 seconds, cooling it to 56 degrees Celsius for 20 seconds, and then heating it to 68 degrees Celsius for one minute. Finally, the sample was heated to [27] 70 degrees Celsius for eight minutes in a process referred to as the final. The PCR products were then analyzed on 1.5 % agarose gels to confirm the presence of the desired DNA sequences and visualized by staining with Safe red dye and photographed under UV transilluminator. ...
... Although there is definite disagreement between Robson's description of the sections and the clades distinguished in this analysis, the association between the sections differ considerably from thoseadvances by Robson. This amended theoryaboutphylogenetic comm unications between major parts within a genus (that is, the clade/grade above) is relevant to the clarification of biogeographic patterns and the reconstruction of historic trait states [27]. ...
Phylogenetic relationships for the widely distributed genus Hypericum (St. John's wort) had already been focused on morphology, with just a few species having been studied molecularly. We're currently in the midst of a long-term study of the genus Hypericum, and we've been analyzing some pretty interesting data. Specifically, we've been looking at the nuclear ribosomal DNA internal transcribed spacer sequences and 28SrRNA of eight different species of Hypericum, as well as one species of Thornea calcicola (which serves as our outgroup). It's all part of our larger effort to better understand the evolutionary relationships between these species. The subsequent parsimony analysis and Bayesian inference support the actual categorization and interspecific affinities. The molecular evidence supports the monophyletic assemblage of the sections in the ITS and 28SrRNA region trees, which indicate four distinct clades with little variation in the positions of the individual species in both trees. We identified traits that support significant clades within the genus Hypericum using Maximum Parsimony and Bayesian approaches to reconstruct ancestral states of the chosen features. The findings support using ITS and 28SrRNA sequencing analysis to clarify the evolutionary connections within the numerous members of the genus Hypericum. It's great to know that the phylogenetic relationships among Hypericum species have been mostly resolved via ITS phylogeny. This means that we have a better understanding of the evolutionary relationships between these species. With this information, we can make more informed decisions about how to classify and study these plants.
... This was followed by 35 cycles of heating the sample to 94 degrees Celsius for 30 seconds, cooling it to 56 degrees Celsius for 20 seconds, and then heating it to 68 degrees Celsius for one minute. Finally, the sample was heated to [27] 70 degrees Celsius for eight minutes in a process referred to as the final. The PCR products were then analyzed on 1.5 % agarose gels to confirm the presence of the desired DNA sequences and visualized by staining with Safe red dye and photographed under UV transilluminator. ...
... Although there is definite disagreement between Robson's description of the sections and the clades distinguished in this analysis, the association between the sections differ considerably from thoseadvances by Robson. This amended theoryaboutphylogenetic comm unications between major parts within a genus (that is, the clade/grade above) is relevant to the clarification of biogeographic patterns and the reconstruction of historic trait states [27]. ...
Phylogenetic hypotheses for the large cosmopolitan genus Hypericum (St. John’s wort) have previously been based on morphology, and molecular studies have thus far included only a few species. In this study, we used Eight taxa representing 2 sections of the genus Hypericum were analyzed along with one representatives of Thornea calcicola using nuclear internal transcribed spacer ITS and 28SrRNA sequences in order to assess the scope of phylogenetic relationships in this species-rich genus. The resulting parsimony analysis and Bayesian Inference corroborates the actual classification and interspecific affinities. The molecular data provide support for the monophyletic assemblage of the sections in both ITS and 28SrRNA regions trees shows four different clades with minimum changes between species location in both tree. We used Maximum Parsimony and Bayesian approach to reconstruct ancestral states of selected characters, which resulted in recognition of characters that support major clades within the genus Hypericum. The obtained results encourage the use of ITS and 28SrRNA sequence analysis in resolving the phylogenetic relationships within the member rich genus Hypericum. Our results differ somewhat from those of recent morphological and cytological studies. The phylogenetic relationships among Hypericum species have now been mostly resolved via 28SrRNA phylogeny.
The present research includes a comparative morphological characters of pollen grains and seeds of (8) cultivars belonging the species Pyrus malus L. namely ("EarlyGold", "GrannySmith", "Royal Cala", "Red Delicious", "Golden Delicious", "Honey Crisp", "Mcintosh", Cox") and (6) Cultivars belonge to the species Pyrus communis L. namely ("Coneference", Decana", "Bonica", "Alkhatuni","Alothmani", "William) which cultivated in northern of Iraq. Were examined by light and scanning electron microscope (SEM). The result of pollen grains showed that pattern tricolporate and the shape in polar view triangular or spherical -triangular or tetrangular but in equatorial view spherical or ovate and surface configuration was striate in all cultivars of the species. The morphological characters of seeds (Shape, Color, Dimension of width and Length, Number of seeds in fruit), in addition to indumentum were found to be diagnostic value for separation cultivars from the two species. In addition to its surface configuration, it was Striate in "EarlyGold", "RoyalCala", "Honey Crisp", "Mcintosh" and "Cox" and Striate papillae in GrannySmith, while the "Coneference", "Decana" and "Bonica, Alkhatuni, and Alothmani had a surface configuration pitted, and Irregular Reticular in Red Delicious and Reticulate papillae in cultivar William, while Golden Deliciou was unique as having an alveolate surface, The quantitative and qualitative morphological characters of pollen grains and seeds were found to be diagnostic value for separation cultivars from the species.
