Statistical comparison between sequencing data and reference genome sequences.

Statistical comparison between sequencing data and reference genome sequences.

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This study investigates the anti-aging effects of various concentrations of blueberry anthocyanins (BA) on the lifespan and health-related phenotypes of Caenorhabditis elegans. Blueberry anthocyanins were administered at concentrations of 50.0 μg/mL, 200.0 μg/mL, and 500.0 μg/mL, and their effects on nematode lifespan, locomotion, pharyngeal pumpin...

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... alignment yielded the following results: the number of reads that mapped to the reference genome (Mapped Reads, MR), the number of reads that mapped uniquely (Unique Mapped Reads, UMR), the number of reads that mapped to the positive strand (Reads Mapped to '+', RM to '+'), and the number of reads that mapped to the negative strand (Reads Mapped to '−', RM to '−'). As shown in Table 4, the alignment rate for all samples exceeded 98%, and the proportion of reads that mapped uniquely was higher than 90%. Additionally, the distribution of reads mapped to the positive and negative strands of the reference genome was about 50% each, indicating high efficiency and accuracy of the alignment, thus confirming the reliability and accuracy of the sequencing results. ...

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