Table 1 - uploaded by Luis Gonzalo Sequeda-Castañeda
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Pentacalia nitida and Pentacalia corymbosa are plant species present in the Colombian páramo regions moorland areas of the Andes Mountains. These plants are employed in popular medicine as an alexiteric, in
edemas or to treat lesions, pharyngitis, sore throat, as a disinfectant, as a scar forming agent, and to alleviate other affections of the skin...
Citations
... Heptasiloxane, hexadecamethyl (4.40%) has been reported for antimicrobial and antioxidant properties (Syeda and Riazunnisa 2020). 4-Hydroxyphenyl acetic acid, ethyl ester (3.88%) has been reported to have properties to treat pulmonary disease (Sequeda-Castañeda et al. 2016). This compound also reported in Populus species (Isidorov and Vinogorova 2003). ...
The methanol extract of Camellia sinensis (MES) and acetone extract of Camellia assamica (AEA) were subjected to the thin layer chromatography to separate the bioactive compounds. The antimicrobial activity of all the fractions was carried out against pathogenic microorganisms by the agar-well diffusion method. The most effective bioactive fraction of each plant species was analysed by GC–MS. Fraction L of methanol extract of C. sinensis (MES) and fraction 5 of acetone extract of C. assamica (AEA) were found very effective against selected pathogenic strains. GC–MS analysis of this fraction showed the presence of n-heptadecanol-1 (68.63%) in MES and 2′,6′dihydroxyacetophenone, bis(trimethylsilyl) (17.58%) in AEA with the highest area. The compounds n-heptadecanol-1 and 2′,6′dihydroxyacetophenone, bis(trimethylsilyl) ether were used for docking to analyse its therapeutic potential. The ligand compound n-heptadecanol-1 (HEP) from MES of C. sinensis and 2′,6′dihydroxyacetophenone, bis(trimethylsilyl) ether from AEA of C. assamica were docked with the target protein dihydropteoate synthase (DHPS) active sites of Escherichia coli and Staphylococcus aureus active sites via Auto Dock Vina, thereby forecasting the finest binding position of ligands. AutoDock Vina docked results revealed the involvement of binding energy for the establishment of the protein–ligand structure complex, besides generating an interpretation of all apparent molecular interactions accountable for its activity. Further, the protein–ligand complex of MES, EcDHPS + HEP and SaDHPS + HEP exhibiting the best binding affinity were − 4.8 kcal/mol and − 3.6 kcal/mol. The protein–ligand complex of AEA, i.e., EcDHPS + DHA and SaDHPS + DHA exhibited the best binding affinity of − 4.8 kcal/mol and − 4.8 kcal/mol.
... When an antioxidant compound is added to the solution of DPPH • radical, it loses its violet, causing the decrease of the initial absorbance causing an inhibition. The conditions for assessing the antioxidant capacity of the extracts were the same of ABTS assay [54,68,70,71]. ...
... Chromatographic peaks were checked for homogeneity with the aid of the mass chromatograms for the characteristic fragment ions and with the help of the peak purity program. Tentative identification criteria of compounds were based on coincidence percentage (≥ 85%) of obtained compounds compared to Wiley 7n.1 and Nist 05a.L mass spectra libraries [71]. ...
Anacardium excelsum is a tree between 15 and 50 meters high, with a thick trunk and is present in some departments of Colombia. Its nut-shaped fruits were used in the old Upar Valley by natives (Valledupar) to make Caracolí bread. Most research has been done at the forest level, but there are few phytochemical and biological activity studies. This bioguided work shows the application of antioxidant methods (ABTS +• , DPPH • and DMPD +•) in the search for active compounds present in the fraction and subfractions of greater activity. The antioxidant capacity was measured to the crude extract in ethanol and the antioxidant activity of the fractions in petroleum ether, dichloromethane, and ethanol-butanol of testa, tegument, flower, fruit, seed, bark, and leaf. Column Chromatography, Thin Layer Chromatography and Gas Chromatography coupled to Mass Spectrometry were performed on the petroleum ether fraction of testa with greater antioxidant capacity, finding the following compounds: 3-pentadec(en)il-phenol, 3-pentadecyl-phenol, 3-heptadeca(dien)il-phenol, 3-heptadec(en)il-phenol, 3-heptadecyl-phenol, 3-nonadec(en)il-phenol, ethyl ester of hexadecanoic acid, ethyl ester of heptadecanoic acid , ethyl ester of linolenic acid, hexadecanoic acid butyl ester, ethyl ether of octadecanoic acid, ethyl ester of (Z)-9-octadecenoic acid, ethyl ester of 9,12-octadecadienoic acid and ethyl ester of eicosanoic acid. The results indicate that Anacardium excelsum is a potential source of bioactive compounds.
