Set up for ultrasound (US) and photoacoustic imaging (PAI) for skin incisions closed with sutures of sealed with LASEs. (a) Prior to imaging, excised skin samples were removed from ice‐cold 1X PBS and placed over a layer of 1.5% agarose low electroendoosmotic (EEO) cooled to room temperature in a 3D printed sample tray. Following this, another layer of agarose solution (~35–45°C) was poured over the skin samples to completely embed the tissues within the agarose layers. The sample tray was then filled with deionized water to form a layer over the agarose layer. (b) (i) Scanning of skin samples were carried out using the Vevo 3100 motor. (ii) The MX550D (50 MHz) linear array transducer and jacket consisting of the fiber bundles (iii) is lowered into the sample tray submerged in water to facilitate opto‐acoustic coupling with a 7 mm standoff from the skin samples (iv). (c) Normalized photoacoustic signal of LASE, suture, and skin sections in the range of 680–960 nm.