Sequences of human primers used in real-time PCR.

Sequences of human primers used in real-time PCR.

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Intestinal epithelial cells (IECs) constitute a defensive physical barrier in mucosal tissues and their disruption is involved in the etiopathogenesis of several inflammatory pathologies, such as Ulcerative Colitis (UC). Recently, the succinate receptor SUCNR1 was associated with the activation of inflammatory pathways in several cell types, but li...

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... expression was analyzed by real-time Quantitative PCR using SYBR ® Ex Taq (Takara Bio Inc., Saint-Germain-en-Laye, France) in LightCycler thermocycler (Roche Diagnostics, Mannheim, Germany). Specific oligonucleotides detailed in Tables 3 and 4 were designed to perform the analysis. The relative gene expression, as fold increase, was expressed as follows: change in expression (fold) = 2 − ∆(∆CT) where ∆CT = CT (target) − CT (housekeeping) and ∆(∆CT) = ∆CT (treated) − ∆CT (control), where β-actin was the housekeeping gene used. ...