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Sequence of the codon-optimized NIE gene (A) along with the amino acid sequence of the NIE protein (B)

Sequence of the codon-optimized NIE gene (A) along with the amino acid sequence of the NIE protein (B)

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Fig. 1: Sequence of the codon-optimized NIE gene (A) along with the...
Fig. 3: Colony PCR of the grown colonies on LB agar supplemented with...
Fig. 4: A. Induction of NIE protein Expression by addition of IPTG....
Fig. 5: Purification of NIE protein using a nickel column. Lane 1:...
Design, Expression and Purification of Strongyloides stercoralis IgG4 Immunoreactive Protein (NIE) in Escherichia coli
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  • Jul 2020
Background: Strongyloidiasis is a public health concern in northern regions of Iran, caused by Strongyloides stercoralis. Auto-infection cycle can be resulted in high parasitic load, especially in immunocompromised hosts. Because of low sensitivity of stool culture and stool-based microscopy techniques, detection of antibodies in patient's sera can...
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