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Figure 4 - An immediate–late gene expression module decodes ERK signal duration

Semi‐quantitative prediction of mRNA log2 fold changes upon different signalling scenarios
Gene expression upon different stimulations was predicted based on fitted model parameters and measured pERK2 levels. Predictions were verified with gene expression time course data.
Signalling input conditions (left side shows deduced input function, and right side shows pERK2 measurements): Sustained ERK signalling (4OHT), 2‐h pulse ERK signalling (4OHT + U0126), growth factor signalling (EGF: epidermal growth factor, FGF: fibroblast growth factor). Deduced input functions: 100% signalling amplitude corresponds to mean induction in training condition (4OHT). Growth factor‐induced input functions are linear interpolations of pERK2 log2 fold changes relative to mean induction in test condition.
Predictions are verified with actual gene expression data. Heat maps show log2 fold changes of induced mRNAs. P: model prediction. D: gene expression data. E: mean error = mean of absolute residuals.
Source data are available online for this figure.
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