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Scutellum, metanotum, metapectal-propodeal complex A Hedychridium calcarium sp. nov., holotype, female; B Hedychridium jucundum, female, from Italy (PRC).
Source publication
Background
DNA barcoding technologies have provided a powerful tool for the fields of ecology and systematics. Here, we present a part of the InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of cuckoo wasps (Hymenoptera, Chrysididae) dataset representing 144 specimens and 103 species, covering approximately 44% of...
Citations
... The existing DNA barcodes of cuckoo wasp species collected in Germany or collected elsewhere but known to occur also in Germany are scattered in the literature and are the result of research projects with very different foci (e.g., addressing specific taxonomic questions, and barcoding faunas outside Germany (e.g., [4,10,[16][17][18][19][20])). Although these barcodes are very valuable, the species identity associated with some of the barcodes deposited in Barcode of Life Data Systems (BOLD, www.boldsystems.org ...
... Additionally, we have included the recently described species Chrysis parabrevitarsis Soon et al. (2021) [10] and acknowledged the recent elevation of Hedychrum rutilans ssp. viridiaureum Tournier (1877) to species status, as proposed by Rosa et al. [18]. Furthermore, we have added Chrysis lanceolata Linsenmaier (1959) to our species list due to its confirmed occurrence in Germany, as indicated by our barcoding efforts. ...
... Hedychrum viridiaureum was previously treated as a subspecies of H. rutilans Dahlbom, 1854 (Figure 3), until Rosa et al. [18] elevated it to species rank. The authors based their decision on the comparatively large divergence (5.3%) of the COI barcode sequences, which formed two BINs, each containing nucleotide sequences that differ very little from each other. ...
Germany is home to a rich cuckoo wasp fauna (Hymenoptera: Chrysididae) with about 108 species. However, several nomenclatural changes, the lack of identification keys, and the discovery of cryptic species difficult to identify based on external morphology have made the identification of several species a challenge. COI barcoding has been instrumental in the identification of some cuckoo wasp species and could help alleviate some of the above problems, but a reliable large reference database containing the cuckoo wasp barcodes is lacking. We present the COI barcodes of more than 800 specimens of 101 cuckoo wasp species native to Germany to lay the foundation for the barcode-based identification of German species. An analysis of the COI barcode sequences suggested groups that are largely consistent with the current taxonomy of the group. We found a few cases of over- or undersplitting of taxa. In some common species, the high degree of barcode divergence suggests the presence of cryptic species that need to be further assessed by integrative approaches. Our library of cuckoo wasp reference barcodes will enhance researchers’ ability to reliably identify species within this fascinating group of insects, in particular for identifying life stages that offer few or no morphological features for species-level identification.
... DNA was extracted from 10 collected specimens using the EasySpin Genomic DNA Microplate Tissue Kit (Citomed, Odivelas, Portugal). DNA barcodes of the Folmer COI region (Folmer et al., 1994) were obtained by amplification of two smaller overlapping regions followed by HTS sequencing, as described in Rosa et al. (2023). Species-specific primers, COI_PPF2a and COI_PPR2b, designed by Puig-Montserrat et al. (2015) were tested in silico using Geneious software R10.2.6 (Kearse et al., 2012) against the produced DNA barcodes to validate complementarity between local C. suppressalis specimens and the designed primers. ...
... This species is possibly a synonym of Stilbum splendidum (Fabricius, 1775), the largest Australian species. However, a revision of the Oriental Stilbum is currently ongoing, and the synonymic list of Stilbum cyanurum (Forster, 1771) as proposed by Kimsey and Bohart ([1990] 1991) clearly includes several valid species, as already recognised by subsequent authors based on morphological characters (eg Mingo 1994; Linsenmaier1997) and DNA barcoding (Rosa et al. 2023). In Australia, at least two valid species are known, Stilbum splendidum (Fabricius, 1775) and S. amethystinum (Fabricius, 1775); two additional Stilbum species are known for Australia: S. chrysocephalum concolor Linsenmaier, 1951, andS. ...
A recent examination of type specimens and unidentified Indian cuckoo wasps deposited at the Natural History Museum (London) and at the Oxford University Museum of Natural History led to the discovery of: two new Indian species, Chrysis parviocula sp. n. (amneris species group) and Elampus bicolor sp. n.; a new synonymy, Chrysis quaerita Nurse, 1902 syn. n. of Chrysis dissimilis Dahlbom, 1854; an unnecessary replacement name, Hedychridium mocsaryi Rosa, 2021 for Hedychridium virescens (Mocsáry, 1914); and a misidentification of Chrysis obscura Smith, 1860 which is now excluded from the Indian fauna. Chrysis perfecta Cameron, 1897 is transferred to the viridissima group. New distributional records are given for Chrysis arkadyi Rosa et al. 2021, Chrysis bayadera du Buysson, 1896, Chrysis musa Semenov-Tian-Shanskij, 1954 and Praestochrysis palawanensis (Mocsáry, 1899). Eighteen types of Indian and Oriental species are illustrated for the first time. Types of six Oriental and Australian species described by Cameron, Smith, and Gray are illustrated and their status and depositories discussed. A lectotype is designated for Chrysis festina Smith, 1874. Type material of Stilbum princeps (Gray, 1832) is rediscovered 190 years after its description.
This paper presents the current list of all species of aculeate wasps (excluding Dryinidae, Bethylidae, Embolemidae) recorded in Germany. This checklist of 592 species forms the basis for the forthcoming revision of the corresponding Red Lists. We also provide German names for all species.
This work presents a taxonomic revision of the Palaearctic types of Chrysididae preserved in the Muséum national d’Histoire naturelle (MNHN), in Paris, with notes on non-Palaearctic types. A total of 854 types are revised, of which 546 are Palaearctic and listed in the present article. The museum houses 257 primary types of Palaearctic taxa and 172 primary types of non-Palaearctic taxa. 109 types of taxa previously considered to be lost or doubtfully deposited in Paris were found and labelled; 11 primary types considered to be deposited in Paris were found in other museums; 10 lectotypes or neotypes were designated in other museums and their paralectotypes are deposited at MNHN. Twenty previously designated lectotypes proved to be invalid and 10 types considered to be preserved in the museum proved to be non-type specimens. Sixteen misinterpretations of type materials are reported. Types spread in seven different collections at MNHN were merged together in a “typothèque” (type collection) stored apart from those deposited in the Abeille de Perrin collection and in the general collection. The neotype of Elampus micans Klug, 1835 is designated, and 38 lectotypes are designated for: Chrysis angustifrons Abeille de Perrin, 1878; C. angustifrons var. castillana du Buysson, 1894; C. aureicollis Abeille de Perrin, 1878; C. aurotecta Abeille de Perrin, 1878; C. barbara Lucas, 1849; C. bidentata var. maculifrons du Buysson, 1895; C. cerastes Abeille de Perrin, 1877; C. dives Lucas, 1849; C. dorsata Brullé, 1833; C. elzearii du Buysson, 1894; C. fugax Abeille de Perrin, 1878; C. gribodoi Abeille de Perrin, 1877; C. incisa Abeille de Perrin & du Buysson, 1877; C. igneola du Buysson, 1896; C. ignita var. rutiliventris Abeille de Perrin, 1879; C. igniventris Abeille de Perrin, 1877; C. laevigata Abeille de Perrin, 1879; C. oraniensis Lucas, 1849; C. phryne Abeille de Perrin, 1878; C. rufitarsis Brullé, 1833; Chrysis uncifera Abeille de Perrin, 1878; C. virgo Abeille de Perrin, 1877; Cleptes abeillei du Buysson, 1887; Elampus bidentatus Lucas, 1849; E. chlorosoma Lucas, 1849; Ellampus araraticus Radoszkowski 1890; E. biaccinctus du Buysson, 1892; Hedychridium minutum var. aereolum du Buysson, 1892; H. minutum var. viridimarginale du Buysson, 1892; H. roseum var. chloropyga du Buysson, 1888; Hedychrum micans Lucas, 1849; Holopyga gloriosa var. aureomaculata Abeille de Perrin, 1879; Notozus productus var. vulgata du Buysson, 1892; Omalus aeneus var. pygialis du Buysson, 1887; O. auratus var. abdominalis du Buysson, 1887; Philoctetes abeillei du Buysson, 1892; P. chobauti du Buysson, 1896; P. tiberiadis Abeille de Perrin & du Buysson, 1887. Five species are revalidated: Chrysis incisa Abeille de Perrin, 1887; C. japanensis Linsenmaier, 1951; C. teilhardi du Buysson, 1908; Hedychridium hispanicum du Buysson, 1892; Pentachryis goliath (Abeille de Perrin, 1878). Haba pygmaea (du Buysson, 1898) n. comb. is raised to species rank and moved to the genus Haba from the genus Holopyga. Isadelphus somalinus Zimmermann, 1952 is transferred to the genus Cephaloparnops Semenow, 1901 n. comb. Chrysis mauritii du Buysson, 1896 is included in the graelsii group; C. sinuosiventris Abeille de Perrin, 1878 is transferred to the elegans group; C. serva du Buysson, 1898a to the bihamata group; C. moriceana du Buysson, 1900 to the maculicornis group. Eleven new synonymies are proposed: Elampus bidentatus Lucas, 1849 n. syn. of Pseudomalus auratus (Linnaeus, 1758); E. particeps du Buysson, 1892 n. syn. of Elampus konowi (du Buysson, 1892); Chrysis bidentata var. intermedia du Buysson, 1887b n. syn. of C. viridula Linnaeus, 1761; C. chevrieri Abeille de Perrin, 1877 n. syn. of C. sybarita Förster, 1853; C. continentalis Linsenmaier, 1959 n. syn. of C. dominula Abeille de Perrin, 1877; C. fulvicornis var. lateralis du Buysson 1900 n. syn. of C. annulata Abeille de Perrin & du Buysson, 1887; C. pseudoincisa Balthasar, 1953 n. syn. of C. incisa Abeille de Perrin & du Buysson, 1887; C. psittacina du Buysson, 1887 n. syn. of C. regina Abeille de Perrin & du Buysson, 1887; Hedychridium minutum aereolum du Buysson in André, 1892 n. syn. of H. ardens (Coquebert, 1801); Hedychrum lucidulum lepeletieri du Buysson, 1898 n. syn. of H. rutilans Dahlbom, 1854; Omalus aeneus var. pygialis du Buysson, 1887 n. syn. of Pseudomalus violaceus (Scopoli, 1763). Additionally, five taxa are considered nomina dubia: Chrysis indica Schrank, 1802; C. mendax Abeille de Perrin, 1878; Ellampus testaceicornis du Buysson, 1892; Hedychrum nanum Chevrier, 1870; Holopyga semiignita Marquet, 1878; four names are considered nomina nuda: Chrysis apicata Abeille de Perrin, 1877, C. delphinalis Giraud (in du Buysson 1899), C. dufouri Abeille de Perrin, 1879 and C. rugosula Abeille de Perrin, 1879; one name is declared as nomen oblitum: Chrysis candens Preyssler, 1791, and one as nomen protectum, Chrysis candens Germar, 1817. The replacement name Chrysis roberti nom. nov. is proposed for C. escalerai du Buysson, 1911, nec García Mercet, 1904. All newly designated lectotypes and types of critical taxonomic cases are illustrated.
In recent years, the research on the Iranian Chrysididae has been extremely prolific, thanks to the efforts of different teams. After the first checklist published by Rosa et al. (2013), more than one hundred taxa of cuckoo wasps have been recorded as new for Iran, including nine taxa described as new for science. Moreover, major modifications impacted the taxonomy of the family with two genera revaluated (Chrysellampus Semenov-Tian-Shanskij, 1932 and Colpopyga Semenov-Tian-Shanskij, 1954), Pseudochrysis Semenow, 1891 reintroduced for Pseudospinolia Linsenmaier, 1951; the description of the genus Morphochrysis Rosa & Pavesi, 2023 and several taxonomical changes to species level which were published and that deeply changed the first checklist, namely. As a consequence of all these fragmented changes, we propose a new, updated checklist of the Iranian species, to summarize all the new findings published in the last years. We describe eleven new species for science, Chrysis amerii Rosa & Farhad, sp. nov., C. chamrosh Rosa, sp. nov., C. crenulata Rosa, sp. nov., C. edentata Rosa & Baiocchi, sp. nov., C. peri Rosa & Baiocchi, sp. nov. and C. titanica Rosa, sp. nov. (succincta group), C. mediasignata Rosa, sp. nov. (leachii group), C. heimi Rosa, sp. nov. (maculicornis group), C. simurgh Rosa, sp. nov. (subsinuata group), Chrysura filidichroa Rosa, sp. nov. (dichroa group) and Hedychridium personatum Rosa, sp. nov. with its own new species-group. We report twenty-six new records of Chrysidinae for Iran: Chrysidea disclusa (Linsenmaier, 1959); Chrysis afghanica Linsenmaier, 1968; C. cylindrica Eversmann, 1858; C. echidna Semenov-Tian-Shanskij, 1967; C. grohmanni bolivari Mercet, 1902; C. klio Balthasar, 1953; C. laetula Semenov-Tian-Shanskij & Nikol'skaya, 1954; C. leuconoe Semenov-Tian-Shanskij, 1967; C. maracandensis Radoszkowski, 1877; C. mirabilis Radoszkovsky, 1877; C. mossulensis Abeille de Perrin-du Buysson, 1887; C. pseudobrevitarsis Linsenmaier, 1951; C. robertsi Rosa, 2020; C. rutilans Olivier, 1791; C. turcomana Semenov-Tian-Shanskij & Nikol'skaya, 1954; Chrysura laodamia laodamia (du Buysson, 1900); Euchroeus pellucidus (Radoszkowski, 1877); Hedychridium bytinskii Linsenmaier, 1959; H. mochii Strumia, 1994; H. plagiatum (Mocsáry, 1883); Hedychrum concinnum (Mocsáry, 1909); H. semicyaneum Mocsáry, 1889; Spinolia stchurovskyi (Radoszkowski, 1877); Spintharina extrema (Semenov-Tian-Shanskij & Nikol’skaya, 1954), and S. houskai (Balthasar, 1953). The current number of known taxa has therefore increased from 185 (179 species and 6 subspecies) to 315 (306 species + 9 subspecies). Hedychrum persicum Mocsáry, 1914 stat. nov. is upgraded to species rank. Chrysis chrysophora Semenow, 1892 and Hedychrum cyaneum Brullé, 1846 are considered nomina dubia and the latter as incertae sedis. Chrysis dawahi Strumia, 2012 is considered nomen nudum. The majority of chrysidid species in Iran (77.64%) are found exclusively in the Western Palearctic region. Among these, 21% are restricted to the Western Palaearctic. A thorough discussion is also provided on unreliable and doubtful species records.
Background
The InBIO Barcoding Initiative (IBI) Dataset - DS-IBILP08 contains records of 2350 specimens of moths (Lepidoptera species that do not belong to the superfamily Papilionoidea). All specimens have been morphologically identified to species or subspecies level and represent 1158 species in total. The species of this dataset correspond to about 42% of mainland Portuguese Lepidoptera species. All specimens were collected in mainland Portugal between 2001 and 2022. All DNA extracts and over 96% of the specimens are deposited in the IBI collection at CIBIO, Research Center in Biodiversity and Genetic Resources.
New information
The authors enabled "The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese moths" in order to release the majority of data of DNA barcodes of Portuguese moths within the InBIO Barcoding Initiative. This dataset increases the knowledge on the DNA barcodes of 1158 species from Portugal belonging to 51 families. There is an increase in DNA barcodes of 205% in Portuguese specimens publicly available. The dataset includes 61 new Barcode Index Numbers. All specimens have their DNA barcodes publicly accessible through BOLD online database and the distribution data can be accessed through the Global Biodiversity Information Facility (GBIF).
All species of the Chrysis angolensis group were synonymised with C. angolensis Radoszkovsky, 1881, excluding Chrysis diademata Mocsáry, 1889, endemic of the Philippines. However, after the study of type materials, four species are herein resurrected: Chrysis callaina Gribodo, 1884 stat. reviv., C. erratica Abeille de Perrin and du Buysson in du Buysson, 1887 stat. reviv., C. mossulensis Abeille de Perrin and du Buysson in du Buysson, 1887 stat. reviv., C. sulcifera Bischoff, 1910 stat. reviv. New synonymies are proposed for the following taxa: C. szalayana Mocsáry, 1912 n. syn. and C. ukerewensis Mocsáry, 1914, n. syn. of C. callaina Gribodo, 1884; Chrysis janthina Smith, 1874 n. syn. of C. brachyceras Bischoff, 1910. The new combination Praestochrysis brachyceras (Bischoff, 1910) n. comb. is proposed. Chrysis fuscipennis Brullé, 1846, the name in use for the oldest taxon described in the angolensis group, was replaced with Chrysis angolensis Radoszkovsky, 1881 because primary junior homonym of C. fuscipennis Dahlbom, 1829. However, Chrysis angolensis is here regarded as nomen dubium, C. fuscipennis Dahlbom, 1829 as nomen oblitum, because no longer in use as a valid name after 1899, and C. fuscipennis Brullé as nomen protectum, thus making the name C. fuscipennis Brullé, 1846 stat. reviv. available and restored.
