Schematic structure of cyr gene clusters from CYN-producing cyanobacterial strains. Gray and white bars, cyr genes; black bar, transposase sequences or vestiges thereof; open triangles, base mutation in this position; solid triangles, nucleotide deletion in this position.  

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... of environmental samples. Water samples were collected in lakes and reservoirs of China from 2006 to 2013 (Table 1). These water bodies were located between 22°N and 47°N in subtropical and temperate regions (see Fig. S1 and Table S2 in the supplemental material). A volume of 300 to 500 ml of water was filtered using a membrane filter (MF- Millipore, 0.22-m pore size) in quadruplicate for each water body at each collection period. The filters were also stored at 80°C before DNA extraction. DNA extraction, PCR, and sequencing. Genomic DNA of cyanobac- ...

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... into an independent clade in phylogenetic trees (data not shown). This clade was separated from the cyr genes of other species by high bootstrap values in the trees of the cyrI and cyrJ genes (97 to 100%). Sequence analysis. The cyr genes of C. raciborskii CHAB358 and R. curvata HB1 were sequenced and assembled into two com- plete gene clusters (Fig. 1). The genome of C. raciborskii CHAB3438 was assembled using high-quality data with an aver- age coverage of 220, and the cyr gene cluster was found to be located in two contigs. The gap was closed by PCR amplification and Sanger sequencing. The final contig had a length of 50,355 bp and contained the whole cyr gene cluster (Fig. 1). The ...

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... gene clusters (Fig. 1). The genome of C. raciborskii CHAB3438 was assembled using high-quality data with an aver- age coverage of 220, and the cyr gene cluster was found to be located in two contigs. The gap was closed by PCR amplification and Sanger sequencing. The final contig had a length of 50,355 bp and contained the whole cyr gene cluster (Fig. 1). The cyr genes in these three gene clusters showed high similarities to those of R. curvata CHAB1150 (99%). The gene arrangement patterns of the cyr gene clusters of the C/R group strains from China were conserved and divergent from that of C. raciborskii AWT205 from Australia. The cyrN and cyrO genes were absent in the cyr gene ...

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... in these three gene clusters showed high similarities to those of R. curvata CHAB1150 (99%). The gene arrangement patterns of the cyr gene clusters of the C/R group strains from China were conserved and divergent from that of C. raciborskii AWT205 from Australia. The cyrN and cyrO genes were absent in the cyr gene clusters of Chinese strains (Fig. ...