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Schematic diagram of phylogenetic analysis, exon/intron distribution, and motifs analysis of SjERF TFs. (A) Phylogenetic tree of 59 SjERF proteins. (B) Motif distribution of 59 SjERF proteins. (C) The exon-intron structure of 59 SjERF genes. Yellow rectangle: UTR; black line: intron; blue rectangle: CDS.

Schematic diagram of phylogenetic analysis, exon/intron distribution, and motifs analysis of SjERF TFs. (A) Phylogenetic tree of 59 SjERF proteins. (B) Motif distribution of 59 SjERF proteins. (C) The exon-intron structure of 59 SjERF genes. Yellow rectangle: UTR; black line: intron; blue rectangle: CDS.

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Introduction Cepharanthine (CEP), a bisbenzylisoquinoline alkaloid (bisBIA) extracted from Stephania japonica, has received significant attention for its anti-coronavirus properties. While ethylene response factors (ERFs) have been reported to regulate the biosynthesis of various alkaloids, their role in regulating CEP biosynthesis remains unexplor...