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Schematic arrangement of the tRNA Tyr /COI positions and the relative regions of the newly designed F and R primers. The numbers show the positions on the Artemia sinica Cai, 1989 mitogenome. The purple line represents the COI barcoding region in metazoan invertebrates (to represent clear positions in this schematic figure, actual scales were not considered).

Schematic arrangement of the tRNA Tyr /COI positions and the relative regions of the newly designed F and R primers. The numbers show the positions on the Artemia sinica Cai, 1989 mitogenome. The purple line represents the COI barcoding region in metazoan invertebrates (to represent clear positions in this schematic figure, actual scales were not considered).

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Article
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Due to the lack of a taxonomic key for the identification of Artemia species, molecular markers have been increasingly used for phylogenetic studies. The mt COI marker is a regularly considered marker for the molecular systematics of Artemia populations. The proposed universal and specific primers have mostly failed to amplify the Artemia aff. sini...

Contexts in source publication

Context 1
... mtCOI barcoding region of Artemia aff. sinica. We hypothesized that, if a forward primer could be designed to start amplification from the joint regions of the last part of the previous gene (tRNA Tyr ) and the beginning of the target mtCOI, the obtained sequence can most probably be referred to an errorless sequence of the mitogenomic COI ( fig. 3). ...
Context 2
... the reverse sequence of tRNA Tyr on the H-strand (bp 1347-1359) and 7 bp of the beginning of COI (bp 1361-1367) were chosen to design the F-primer. Then, based on the barcoding region of the COI marker ( Folmer et al., 1994), a 21-bp-long fragment was chosen on the mitogenome of A. sinica (MK069595), from bp 2139 to 2160, to design the R-primer ( fig. 3). A novel primer ...
Context 3
... of the COI marker in other bisexual species and in parthenogenetic populations of Artemia. The list of studied species and populations has been summarized in table I. We thus designed the forward and reverse primers, ArtCOI-F and ArtCOI-R, that completely cover the COI barcoding region for metazoan invertebrates (Folmer et al., 1994) (see fig. ...