SUCNR1 and inflammasome components are increased and positively correlated in surgical resections from UC patients. (a) Graphs show mRNA expression of SUCNR1, NLPR3, CASP-1, IL1B, IL18 and ASC in intestinal resections from UC patients (n = 25) and non-IBD patients (n = 30). (b) Graphs show protein expression of SUCNR1, NLRP3 and Caspase-1/pro-Caspase-1 (n = 15). Bars in graphs represent mean ± SEM. * p < 0.05 vs. non-IBD patients. (c) Graphs show the correlations between data relative to mRNA expression of SUCNR1 vs. mRNA expression of the inflammasome components NLRP3, CASP1, IL1B and IL18 (expressed as ΔCt). In each correlation, the value of the Spearman's correlation coefficient (R) and p value are shown.

SUCNR1 and inflammasome components are increased and positively correlated in surgical resections from UC patients. (a) Graphs show mRNA expression of SUCNR1, NLPR3, CASP-1, IL1B, IL18 and ASC in intestinal resections from UC patients (n = 25) and non-IBD patients (n = 30). (b) Graphs show protein expression of SUCNR1, NLRP3 and Caspase-1/pro-Caspase-1 (n = 15). Bars in graphs represent mean ± SEM. * p < 0.05 vs. non-IBD patients. (c) Graphs show the correlations between data relative to mRNA expression of SUCNR1 vs. mRNA expression of the inflammasome components NLRP3, CASP1, IL1B and IL18 (expressed as ΔCt). In each correlation, the value of the Spearman's correlation coefficient (R) and p value are shown.

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Intestinal epithelial cells (IECs) constitute a defensive physical barrier in mucosal tissues and their disruption is involved in the etiopathogenesis of several inflammatory pathologies, such as Ulcerative Colitis (UC). Recently, the succinate receptor SUCNR1 was associated with the activation of inflammatory pathways in several cell types, but li...

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Context 1
... we analyzed the expression of SUCNR1 and inflammasome components in surgical resections obtained from UC patients. As shown in Figure 6a,b, gene and protein expression of SUCNR1 was significantly increased in UC patients compared with non-IBD patients. In parallel, the mRNA expression of NLRP3, CASP-1 and IL1B was significantly higher in samples from UC patients than in control tissue, whereas no differences were detected in the expression of ASC nor IL18 (Figure 6a). ...
Context 2
... shown in Figure 6a,b, gene and protein expression of SUCNR1 was significantly increased in UC patients compared with non-IBD patients. In parallel, the mRNA expression of NLRP3, CASP-1 and IL1B was significantly higher in samples from UC patients than in control tissue, whereas no differences were detected in the expression of ASC nor IL18 (Figure 6a). The ratio of Caspase-1/pro-Caspase-1 was significantly increased in resections from UC patients, as well as NLRP3 protein levels (Figure 6b). ...
Context 3
... parallel, the mRNA expression of NLRP3, CASP-1 and IL1B was significantly higher in samples from UC patients than in control tissue, whereas no differences were detected in the expression of ASC nor IL18 (Figure 6a). The ratio of Caspase-1/pro-Caspase-1 was significantly increased in resections from UC patients, as well as NLRP3 protein levels (Figure 6b). Next, we wanted to elucidate whether the succinate receptor was associated with the inflammasome activation in human intestinal mucosa of UC patients. ...
Context 4
... we wanted to elucidate whether the succinate receptor was associated with the inflammasome activation in human intestinal mucosa of UC patients. Results revealed a positive and significant correlation between the expressions of SUCNR1 and NLRP3, CASP1, IL1B, IL18 and ASC (Figure 6c.) ...