SBP-protein applications. Panel (A): use of SBPs for protein purification. (Left) Schematic illustration depicting the rapid and efficient purification of GFP-Car9 using a silica bead column via FPLC. SDS-PAGE results showing the elution of GFP-SBP using a lysine-containing buffer. (Right) Fraction of free protein (GFP and GFP-Car9) after incubation with silica gel beads, demonstrating the efficient attachment of the protein to silica via the Car9 modification. Reprinted from [18] John Wiley & Sons. © 2014 Wiley Periodicals, Inc. Panel (B): use of SBPs for biocatalysis in bioproduction. (Left) Illustration of the TAL-SiBP2 construct and its role in producing p-Coumaric acid. (Right) Reusability of immobilized TAL-SiBP2 under alkaline conditions, evaluated as the percentage of enzyme activity relative to the number of system uses. Reprinted from [170], Copyright (2022), with permission from Elsevier. Panel (C): use SBPs for bioremediation. (Left) Scheme of the SUP-SBP construct used for capturing uranyl ions. (Right) Reusability of the system. Reprinted with permission from [173]. Copyright (21) American Chemical Society.