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Relative gene expression of IL-6, IL-8, and TNF-α upon infection with HAdV5, HAdV26, or HAdV35 in SK-OV-3 cells with decreased expression of αvβ3 integrin. (A,B) Expression of αvβ3 integrin in SK-OV-3 cells after downregulating expression of β3 integrin using si(β3)_1 and si(β3)_2, respectively. Thin-line histograms represent isotype controls (IgG) and filled histograms the expression of αvβ3 integrin. Representative data from two independent experiments yielding similar results are shown (n = 2). (C,E,G) Relative gene expression of IL-6, IL-8, and TNF-α upon infection with HAdV5, HAdV26, or HAdV35 in SK-OV-3 cells transfected with si(β3)_1. (D,F,H) Relative gene expression of IL-6, IL-8, and TNF-α upon infection with HAdV5, HadV26, or HAdV35 in SK-OV-3 cells transfected with si(β3)_2. Cells transfected with si(β3)_1 or si(β3)_2 were transduced with 10,000 vp/cell of virus for 1 h. Relative gene expression was determined by RT-qPCR. Data are shown as mRNA levels relative to the cells transfected with scrambled siRNA (si(-)), plus standard deviations. Representative data from three independent experiments yielding similar results are shown (n = 3). * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns = non-significant (p > 0.05).

Relative gene expression of IL-6, IL-8, and TNF-α upon infection with HAdV5, HAdV26, or HAdV35 in SK-OV-3 cells with decreased expression of αvβ3 integrin. (A,B) Expression of αvβ3 integrin in SK-OV-3 cells after downregulating expression of β3 integrin using si(β3)_1 and si(β3)_2, respectively. Thin-line histograms represent isotype controls (IgG) and filled histograms the expression of αvβ3 integrin. Representative data from two independent experiments yielding similar results are shown (n = 2). (C,E,G) Relative gene expression of IL-6, IL-8, and TNF-α upon infection with HAdV5, HAdV26, or HAdV35 in SK-OV-3 cells transfected with si(β3)_1. (D,F,H) Relative gene expression of IL-6, IL-8, and TNF-α upon infection with HAdV5, HadV26, or HAdV35 in SK-OV-3 cells transfected with si(β3)_2. Cells transfected with si(β3)_1 or si(β3)_2 were transduced with 10,000 vp/cell of virus for 1 h. Relative gene expression was determined by RT-qPCR. Data are shown as mRNA levels relative to the cells transfected with scrambled siRNA (si(-)), plus standard deviations. Representative data from three independent experiments yielding similar results are shown (n = 3). * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns = non-significant (p > 0.05).

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