Figure - available from: Applications in Plant Sciences
This content is subject to copyright. Terms and conditions apply.
Source publication
Premise:
Within a broader study on leaf fossilization in freshwater environments, a long-term study on the development and microbiome composition of biofilms on the foliage of aquatic plants has been initiated to understand how microbes and biofilms contribute to leaf decay and preservation. Here, water lily leaves are employed as a study model to...
Similar publications
Classical biological control aims to suppress alien invasive plant populations by introducing host-specific natural enemies from the native range. This relies on the assumption that invasive plant populations in the invaded range benefit from the release of natural enemies. Pre-release surveys in the invaded range are a useful way to determine if e...
Key Message
The first record of gene expression during seed development within the Nymphaeales provides evidence for a variety of biological processes, including dynamic epigenetic patterning during sexual reproduction in the water lily Nymphaea thermarum.
Abstract
Studies of gene expression during seed development have been performed for a growin...
Nymphaea hybrid, a precious water lily, is a widely-cultivated aquatic flower with high ornamental, economic, medicinal, and ecological value; it blooms recurrently and emits a strong fragrance. In the present study, in order to understand the volatile components of N. hybrid and its relationship with petals structure characteristics, the morpholog...
Nymphaeaceae are early diverging angiosperms with large flowers characterized by showy petals and stamens not clearly whorled but presenting a gradual morphological transition from the outer elements to the inner stamens. Such flower structure makes these plant species relevant for studying flower evolution. MADS-domain transcription factors are cr...
The genus Nymphaea L. (water lily) is the most diverse genus in the family Nymphaeaceae, with more than 50 species worldwide, including 11 species distributed in Africa. The complex and variable morphology of Nymphaea makes it extremely difficult to accurately identify species based on morphological characteristics alone. DNA barcoding has the pote...
Citations
... In this study, species of the genera Tolumonas, Aeromonas, Vogesella, Rhodoferax, Sphaerotilus, Idionella, Paucibacter, Aquabacterium, Undibacterium, and Flectobacillus were potential biofilm formers. The genus Aeromonas is a strong decomposer of soft tissues as well as a biofilm former 24 . These facultatively anaerobic bacteria were observed in high abundance during the initial stages of the experiment in all plant biofilm samples. ...
... The total genomic DNA of each sample was extracted following the manufacturer's protocol using a Fast DNA spin kit for soil (MP Biomedicals), which had been found to be optimal for the extraction of DNA from biofilms on decaying leaves 24 www.nature.com/scientificreports/ DNA was eluted in 50 µl Dnase/RNase-free water and the concentration of the extracted nucleic acids was measured using a Qubit 4.0 fluorometer (Thermo Fisher Scientific, USA) with the dsDNA HS assay kit (Invitrogen). ...
Biofilms are important in the natural process of plant tissue degradation. However, fundamental knowledge of biofilm community structure and succession on decaying leaves under different oxygen conditions is limited. Here, we used 16S rRNA and ITS gene amplicon sequencing to investigate the composition, temporal dynamics, and community assembly processes of bacterial and fungal biofilms on decaying leaves in vitro. Leaves harvested from three plant species were immersed in lake water under aerobic and anaerobic conditions in vitro for three weeks. Biofilm-covered leaf samples were collected weekly and investigated by scanning electron microscopy. The results showed that community composition differed significantly between biofilm samples under aerobic and anaerobic conditions, though not among plant species. Over three weeks, a clear compositional shift of the bacterial and fungal biofilm communities was observed. The alpha diversity of prokaryotes increased over time in aerobic assays and decreased under anaerobic conditions. Oxygen availability and incubation time were found to be primary factors influencing the microbial diversity of biofilms on different decaying plant species in vitro. Null models suggest that stochastic processes governed the assembly of biofilm communities of decaying leaves in vitro in the early stages of biofilm formation and were further shaped by niche-associated factors.
... form biofilms, particularly in association with heart valve infection (endocarditis), various types of surgical implants, and by potentially localizing within other sites in the body, such as bone (osteomyelitis) or the oral cavity (76)(77)(78)(79)(80)(81)(82)(83)(84). Unfortunately, when bacterial biofilms are present, extraction of high-quality nucleic acids for molecular analysis presents specific challenges (85)(86)(87)(88)(89)(90)(91)(92)(93). If, or the extent to which, biofilm formation could adversely influence culture isolation, or Bartonella spp. ...
Bacteria of the genus Bartonella, a member of the Alphaproteobacteria, are fastidious, Gram-negative, aerobic bacilli that comprise numerous species, subspecies, and genotypes. Bartonella henselae, with a worldwide distribution, infects cats, dogs, horses, humans, and other mammals. Diagnostically, direct detection of Bartonella henselae in patient blood specimens by culture or molecular methods is required to confirm infection with this bacterium. Enrichment blood culture combined with quantitative PCR (qPCR) or ddPCR enhances the sensitivity of direct detection. The addition of sheep blood to liquid culture media increased the Bartonella henselae DNA concentration compared to controls, additionally improving PCR direct detection sensitivity.
IMPORTANCE This study aims to improve diagnostic detection of Bartonella henselae. Patient samples are combined with enriched bacterial cultures aimed at growing Bartonella henselae for the best possible chance at detection. However, current Bartonella growth methods could be improved. The DNA extraction method used by most laboratories should also be optimized. Sheep blood was added to increase the growth of Bartonella henselae and multiple DNA extraction methods were to be compared to each other.
... For leaf samples, one sample was collected from the top (15 cm) of three to five plants of each species at each site. Approximately 10 g (fresh weight) of leaves were transferred to sterile 500 mL polyethylene bottles containing 400 mL of 50 mM phosphate-buffered saline (PBS, pH = 7.4) solution Xia et al., 2020) and subsequently underwent 3 min of ultrasonic treatment in an ultrasonic cleaner bath, 30 min in a shaker (225 r min −1 ), and a further 3 min of ultrasonic treatment (Xian et al., 2020;Janssen et al., 2021). The suspension was then filtered through 0.22-μm membrane filters to collect epiphytic bacteria. ...
In aquatic ecosystems, large amounts of epiphytic bacteria living on the leaf surfaces of submerged macrophytes play important roles in affecting plant growth and biogeochemical cycling. The restoration of different submerged macrophytes has been considered an effective measure to improve eutrophic lakes. However, the community ecology of epiphytic bacteria is far from well understood for different submerged macrophytes. In this study, we used quantitative PCR, 16S rRNA gene high-throughput sequencing and functional prediction analysis to explore the structure and function of epiphytic bacteria in an aquatic ecosystem recovered by three submerged macrophytes (Hydrilla verticillata, Vallisneria natans and Potamogeton maackianus) during two growth periods. The results showed that the community compositions and functions of epiphytic bacterial communities on the submerged macrophyte hosts were different from those of the planktonic bacterial communities in the surrounding water. The alpha diversity of the epiphytic bacterial community was significantly higher in October than in July, and the community compositions and functions differed significantly in July and October. Among the three submerged macrophytes, the structures and functions of the epiphytic bacterial community exhibited obvious differences, and some specific taxa were enriched on the biofilms of the three plants. The alpha diversity and the abundance of functions related to nitrogen and phosphorus transformation were higher in the epiphytic bacteria of P. maackianus. In summary, these results provide clues for understanding the distribution and formation mechanisms of epiphytic bacteria on submerged macrophyte leaves and their roles in freshwater ecosystems.
Earthworms play an important role in the soil environment. To explore the difference in earthworms influence on various media in different soil-plant systems, the abundance of tetracycline, sulfonamide and quinolone resistance genes and the structure of the bacterial community were analysed from five different media including non-rhizosphere soil, rhizosphere soil, phyllosphere, root endophytes and earthworm intestine by real-time quantitative PCR and high-throughput 16S rRNA sequencing. Studies have shown that earthworms can reduce the absolute abundance of antibiotic resistance genes (ARGs) in non-rhizosphere soil. Root endophytes in the soil-cabbage system and rhizosphere soil in the soil-setaria system had the same findings. Earthworms can change the bacterial community structure, especially that of Proteobacteria and Cyanobacteria in the phyllosphere and root endophytes. Redundancy analysis (RDA) results that bacterial community change was the main factor affecting ARGs. In addition, earthworms increased the proportion of Cyanobacteria in root endophytes, and Cyanobacteria was significantly positively correlated with sul3. This study provides a scientific basis for controlling the migration and diffusion of ARGs and reducing environmental risks in soil-plant systems in the future.
