Figure 4 - NAD+ capping of RNA in Archaea and Mycobacteria

Figure 4. RNAP from M. smegmatis incorporates NAD + caps into Ms1 in vitro. Abortive transcription was used to monitor the relative affinities of ATP and NAD + as initiating substrates for RNAP at the Ms1 promoter (shown on top). The +1A and +2C positions are shown in bold in the red box. Representative primary data show the abortive transcript (NAD + -C*) formed by NAD + and the radiolabelled CTP (C*). The presence/absence of ATP and NAD + (each 200 µM) is indicated. The dashed line indicates that the lanes from the same gel were electronically assembled for presentation. The graph shows the quantification of the NAD + -C* dinucleotide, normalized to the transcription signal in the absence of ATP, which was set as 1 (lane 3). The bars show average values ± SD, n=3.

RNAP from M. smegmatis incorporates NAD + caps into Ms1 in vitro. Abortive transcription was used to monitor the relative affinities of ATP and NAD + as initiating substrates for RNAP at the Ms1 promoter (shown on top). The +1A and +2C positions are shown in bold in the red box. Representative primary data show the abortive transcript (NAD + -C*) formed by NAD + and the radiolabelled CTP (C*). The presence/absence of ATP and NAD + (each 200 µM) is indicated. The dashed line indicates that the lanes from the same gel were electronically assembled for presentation. The graph shows the quantification of the NAD + -C* dinucleotide, normalized to the transcription signal in the absence of ATP, which was set as 1 (lane 3). The bars show average values ± SD, n=3.

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Reference

NAD+ capping of RNA in Archaea and Mycobacteria - Scientific Figure on ResearchGate. Available from: https://www.researchgate.net/figure/RNAP-from-M-smegmatis-incorporates-NAD-caps-into-Ms1-in-vitro-Abortive-transcription_fig2_357091208 [accessed 21 Jun 2025]

Caption

Figure 4. RNAP from M. smegmatis incorporates NAD + caps into Ms1 in vitro. Abortive transcription was used to monitor the relative affinities of ATP and NAD + as initiating substrates for RNAP at the Ms1 promoter (shown on top). The +1A and +2C positions are shown in bold in the red box. Representative primary data show the abortive transcript (NAD + -C*) formed by NAD + and the radiolabelled CTP (C*). The presence/absence of ATP and NAD + (each 200 µM) is indicated. The dashed line indicates that the lanes from the same gel were electronically assembled for presentation. The graph shows the quantification of the NAD + -C* dinucleotide, normalized to the transcription signal in the absence of ATP, which was set as 1 (lane 3). The bars show average values ± SD, n=3.

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<a href="https://www.researchgate.net/figure/RNAP-from-M-smegmatis-incorporates-NAD-caps-into-Ms1-in-vitro-Abortive-transcription_fig2_357091208"><img src="https://www.researchgate.net/publication/357091208/figure/fig2/AS:1101635887603712@1639661871051/RNAP-from-M-smegmatis-incorporates-NAD-caps-into-Ms1-in-vitro-Abortive-transcription.jpg" alt="RNAP from M. smegmatis incorporates NAD + caps into Ms1 in vitro. Abortive transcription was used to monitor the relative affinities of ATP and NAD + as initiating substrates for RNAP at the Ms1 promoter (shown on top). The +1A and +2C positions are shown in bold in the red box. Representative primary data show the abortive transcript (NAD + -C*) formed by NAD + and the radiolabelled CTP (C*). The presence/absence of ATP and NAD + (each 200 µM) is indicated. The dashed line indicates that the lanes from the same gel were electronically assembled for presentation. The graph shows the quantification of the NAD + -C* dinucleotide, normalized to the transcription signal in the absence of ATP, which was set as 1 (lane 3). The bars show average values ± SD, n=3."/></a>