Figure 1 - An evaluation of methods for extraction and quantification of protein from marine macro- and microalgae

Quantification of protein in three species of marine macroalgae (A) Porphyra acanthophora var. acanthophora , (B) Sargassum vulgare and (C) Ulva fasciata by the Lowry (Lwy) and Bradford (Bdf) methods, using bovine serum albumin (BSA) and casein (CAS) as protein standards in the calibration curves. Three variables evaluated: (i) the water volume used to incubate samples (1 and 4 mL); (ii) the length of the incubation period for the extraction of protein (6 and 12 h); and (iii) the use of a Potter homogenizer for grinding samples. All assays included the precipitation of protein with 25% TCA, in a proportion of 2.5:1 (TCA:homogenate). Mean ± S . D . ( n = 6).

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An evaluation of methods for extraction and quantification of protein from marine macro- and microalgae - Scientific Figure on ResearchGate. Available from: https://www.researchgate.net/figure/Quantification-of-protein-in-three-species-of-marine-macroalgae-A-Porphyra-acanthophora_fig1_226885535 [accessed 17 Jan 2025]

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Figure 1 . Quantification of protein in three species of marine macroalgae (A) Porphyra acanthophora var. acanthophora , (B) Sargassum vulgare and (C) Ulva fasciata by the Lowry (Lwy) and Bradford (Bdf) methods, using bovine serum albumin (BSA) and casein (CAS) as protein standards in the calibration curves. Three variables evaluated: (i) the water volume used to incubate samples (1 and 4 mL); (ii) the length of the incubation period for the extraction of protein (6 and 12 h); and (iii) the use of a Potter homogenizer for grinding samples. All assays included the precipitation of protein with 25% TCA, in a proportion of 2.5:1 (TCA:homogenate). Mean ± S . D . ( n = 6).

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<a href="https://www.researchgate.net/figure/Quantification-of-protein-in-three-species-of-marine-macroalgae-A-Porphyra-acanthophora_fig1_226885535"><img src="https://www.researchgate.net/profile/Elisabete-Barbarino/publication/226885535/figure/fig1/AS:302295502868493@1449084272723/Quantification-of-protein-in-three-species-of-marine-macroalgae-A-Porphyra-acanthophora.png" alt="Quantification of protein in three species of marine macroalgae (A) Porphyra acanthophora var. acanthophora , (B) Sargassum vulgare and (C) Ulva fasciata by the Lowry (Lwy) and Bradford (Bdf) methods, using bovine serum albumin (BSA) and casein (CAS) as protein standards in the calibration curves. Three variables evaluated: (i) the water volume used to incubate samples (1 and 4 mL); (ii) the length of the incubation period for the extraction of protein (6 and 12 h); and (iii) the use of a Potter homogenizer for grinding samples. All assays included the precipitation of protein with 25% TCA, in a proportion of 2.5:1 (TCA:homogenate). Mean ± S . D . ( n = 6)."/></a>