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Pure colonies of Malassezia pachydermatis isolated on BD Sabouraud Agar with Chloramphenicol and Cycloheximide. Nail fragments were obtained from a case of onychomycosis in a dog.
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Background: Malassezia species are commensal skin organisms of warm-blooded vertebrates that can act as opportunistic pathogens. Malassezia pachydermatis is of importance in both veterinary and human medicine. Recognised initially as causes of infection of the skin, they are now known to be superficial commensals as well as potential causes of infe...
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Context 1
... fragments were cultured onto Sabouraud Dextrose Agar with Chloramphenicol and Cycloheximide 1 . After 10 days of incubation (32ºC) the mycological culture of nail fragments allowed the isolation of pure colonies of Malassezia pachydermatis (Figure 1). The histopathological evaluation was performed by Sector of Veterinary Clinical Pathology, Faculdade de Veterinária (FAVET/UFRGS) and fungal infection due to Malassezia pachydermatis was confirmed by histopathologic examination (Hematoxylin and eosin and Grocott's methenamine silver stain) of the nail (Figure 2), that revealed abundant yeasts (blastoconidia). ...