Pulsed isoflurane protocol. “On-target” trials of muscimol microinjection into the mesopontine tegmentum are those which extended anesthesia time beyond that attributable to residual circulating isoflurane (ISO). These define the location of the MPTA. A, B Meanings of symbols and lines are given in the legend for Fig. 2. Symbol shapes in B indicate the volume of muscimol microinjected in individual trials in the mouse indicated below. All 21 mice are BALB/c females

Pulsed isoflurane protocol. “On-target” trials of muscimol microinjection into the mesopontine tegmentum are those which extended anesthesia time beyond that attributable to residual circulating isoflurane (ISO). These define the location of the MPTA. A, B Meanings of symbols and lines are given in the legend for Fig. 2. Symbol shapes in B indicate the volume of muscimol microinjected in individual trials in the mouse indicated below. All 21 mice are BALB/c females

Source publication
Article
Full-text available
The mesopontine tegmental anesthesia area (MPTA) was identified in rats as a singular brainstem locus at which microinjection of minute quantities of GABAergic agents rapidly and reversibly induces loss-of-consciousness and a state of general anesthesia, while lesioning renders animals insensitive to anesthetics at normal systemic doses. Obtaining...