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Fig 3 - Identification of Neuroactive Constituents of the Ethyl Acetate Fraction from Cyperi Rhizoma Using Bioactivity-Guided Fractionation

Fig. 3. Protective effects of constituents 1-9 against neurotoxins in in vitro. (A) Cells were treated with constituents 1-9 (10 μM) for 1 h and incubated with 6-OHDA (100 μM) for a further 3 h. Cell viabilities are expressed as a percentage of the controls (cells treated with vehicle for 4 h). (B) Cells were treated with constituents 1-9 (10 μM) for 1 h and incubated with corticosterone (100 μM) for a further 48 h. Cell viabilities are expressed as a percentage of the controls (cells treated with vehicle for 49 h). Values are indicated as the mean ± SEM. ***p<0.001; mean values were significantly different from the control group. # p<0.05, ## p<0.01 and ### p<0.001; mean values were significantly different from the 6-OHDA or corticosterone only treated group. 
Protective effects of constituents 1-9 against neurotoxins in in vitro. (A) Cells were treated with constituents 1-9 (10 μM) for 1 h and incubated with 6-OHDA (100 μM) for a further 3 h. Cell viabilities are expressed as a percentage of the controls (cells treated with vehicle for 4 h). (B) Cells were treated with constituents 1-9 (10 μM) for 1 h and incubated with corticosterone (100 μM) for a further 48 h. Cell viabilities are expressed as a percentage of the controls (cells treated with vehicle for 49 h). Values are indicated as the mean ± SEM. ***p<0.001; mean values were significantly different from the control group. # p<0.05, ## p<0.01 and ### p<0.001; mean values were significantly different from the 6-OHDA or corticosterone only treated group. 
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