Figure - available from: Frontiers in Physiology
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Proportion of control-normalized survival in Trial 1 (A) and Trial 2 (B) and normal development in Trial 1 (C) and Trial 2 (D) plotted against copper concentration. Mean survival with standard error (A,B) and mean normal development with standard error and modeled 4-parameter log-logistic curves (C,D) are plotted. Blue points and lines represent control-normalized survival (A,B) and normal development (C,D), while the black dashed line represents non-normalized normal development. Asterisks indicate concentrations that exhibited significantly different proportions from the control (p < 0.005). The normal development EC50 was 5.87 μg/L for the pooled larvae trial (Trial 1), and 6.43 μg/L for the single larvae trial (Trial 2).
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One of the challenges facing efforts to generate molecular biomarkers for toxins is distinguishing between markers that are indicative of exposure and markers that provide evidence of the effects of toxicity. Phenotypic anchoring provides an approach to help segregate markers into these categories based on some phenotypic index of toxicity. Here we...
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... Following a 10-min incubation at room temperature, the beads were bound to a magnet, washed twice with 80% ethanol and dried for 10 min. The dried bead-bound total RNA was used directly as input to prepare 3′-tag RNAseq libraries using a protocol adapted from a single-cell RNAseq library construction protocol (Hall and Gracey 2021). Briefly, the bead-bound RNA was resuspended in 8 μL of a reverse-transcription reaction mixture with each well receiving a unique indexed anchored oligo-dT primer that contained the Illumina p7 sequence. ...
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