Figure - available from: Journal of Chemistry
This content is subject to copyright. Terms and conditions apply.
Source publication
An accurate and rapid reverse HPLC method has been developed and validated for the simultaneous quantification of lamivudine, nevirapine, and tenofovir disoproxil fumarate. Suitable separation was achieved on Phenomenex Synergi C18 (250 × 4.6 mm, 4 μm) using mobile phase, methanol (50%): ammonium acetate buffer (adjusted to pH 2.80) (40%): acetonit...
Similar publications
Background: The multidrug resistance protein, MDR1 is involved in the transport of numerous drugs. Polymorphism of MDR1 is linked with the treatment outcome. Antiretroviral (ARV) regimen is being used to manage the progression of HIV infection. Ethnic disparities have been observed in the distribution of MDR1 genotypes.
Methods: MDR1 polymorphisms...
Background:
We analysed the impact of breastfeeding, antiretroviral drugs and health service factors on cumulative (6 weeks to 18 months) vertical transmission of HIV (MTCT) and 'MTCT-or-death', in South Africa, and compared estimates with global impact criteria to validate MTCT elimination: (1) <5% final MTCT and (2) case rate ≤50 (new paediatric...
Citations
... (1) Specificity/Selectivity. is was demonstrated by comparing the chromatograms from the diluent (mobile phase system) with that from the working standards at a concentration level of 80 μg/mL for quinidine, cinchonine, cinchonidine, dihydroquinine, dihydroquinidine, and cryptolepine (100% working) and 400 μg/mL for quinine sulfate [22]. ...
Quinine- and cryptolepine-based antimalarials serve as valuable alternatives to artemisinin-based combination therapies (ACTs) in Ghana. Their use, however, is associated with adulteration and substandard quality challenges. An HPLC method targeting quinoline and indoloquinoline antimalarial alkaloids was developed, validated, and applied to evaluate herbal and pharmaceutical antimalarial formulations (HPAFs) and starting materials (APIs). The separation/quantitation of the alkaloids (including quinine, quinidine, cinchonine, cinchonidine, dihydroquinine, dihydroquinidine, and cryptolepine) was achieved on a Zorbax SB-CN column (250 mm × 4.6 mm, 5 μm), with an isocratic elution system of methanol: trifluoroacetic acid (0.1%, v/v) (15 : 85, v/v) at 1.5 mL/min and 223 nm. Method validation was according to ICH Q2(R1) guidelines. It was then used to assess the quality of APIs (n = 3) and HPAFs (n = 44) including quinine-based pharmaceutical antimalarial formulations (QBPAFs) (n = 23) and herbal antimalarial products (HAMPs). The method was found to be specific, selective, accurate, precise, and robust toward the alkaloids with linearity achieved within specified concentration ranges (r2 > 0.995 for all analytes). Analyte stability ranged between 6 and 12 hours. All the APIs contained quinine
... Method validation. The developed method was validated in accordance with the ICH Guideline Q2(R1) [21] ; following recommendations from literature [22][23][24] . ...
... Specificity and selectivity. Specificity and selectivity were assessed by comparing the chromatogram from a matrix without expected analytes with that of a matrix containing the expected analytes (that is, 1.2 mg/mL of PSD and 0.08 mg/mL of CPM) [24] . The selectivity was confirmed by comparing the Mean ± SD of the retention times for the analytes using Student t -test analysis [22] . ...
Common cold medicines usually present as multi-component products and constitute one of the largest groups of ‘over-the-counter’ medicines marketed in Ghana. Due to their wide patronage and easy accessibility, their quality control is of utmost importance to medicine consumers. In this study, the Design of Experiment (DOE) concept is adopted to develop a chromatographic method for the simultaneous assay of pseudoephedrine hydrochloride and chlorphenamine maleate in commercially available products. The separation of the drugs was achieved on an Agilent Eclipse Plus C18 analytical column (4.6 × 150 mm; 5 µm) and detected at 252 nm, while maintaining column temperature at 30 oC. The method parameters, including acetonitrile concentration in the mobile phase, mobile phase pH and flow rate were optimized using a Central Composite Design study, while monitoring the method attributes, asymmetric factors, selectivity, retention times, and resolution. The outcome showed a good correlation between experimental and predictive values throughout the modeled Design Space, with a Desirability of 1.000. The conditions optimized included a mobile phase containing acetonitrile, 50 mM aqueous acetate buffer solution (pH = 3.19) and triethylamine (15.34:84.65:0.01 v/v/v), and a flow rate of 1.437 mL/min. The method was then validated following the International Council for Harmonization Q2(R) guidelines to establish linearity and range, accuracy, precision, robustness, and stability of the test solution. The developed method was successfully applied to estimate pseudoephedrine hydrochloride and chlorphenamine maleate contents in single and fixed-dose formulations commercially available (N = 38).
