Figure 4 - uploaded by Phuc Van Pham
Content may be subject to copyright.
Primary cells derived from tumor fragments cultured in Medium DB. Primary cells began to migrate from tumuor fragments and proliferated rapidly with mesenchymal-like cells with a small nucleus and elongated shape, and epithelial-like cells with a bean shape and the large nucleus with 4 different samples (A-D).

Primary cells derived from tumor fragments cultured in Medium DB. Primary cells began to migrate from tumuor fragments and proliferated rapidly with mesenchymal-like cells with a small nucleus and elongated shape, and epithelial-like cells with a bean shape and the large nucleus with 4 different samples (A-D).

Source publication
Article
Full-text available
Breast cancer cells from patients hold an important role in antigen production for immunotherapy, drug testing, and cancer stem cell studies. To date, although many studies have been conducted to develop protocols for the isolation and culture of breast cancer cells from tumour biopsies, the efficiencies of these protocols remain low. This study ai...

Similar publications

Article
Full-text available
Understanding the regulatory mechanisms unique to breast cancer stem cells (BCSCs) is required to control breast cancer metastasis. We found that phthalates promote BCSCs in human breast cancer cell cultures and xenograft tumors. A toxic phthalate, benzyl butyl phthalate (BBP), activated aryl hydrocarbon receptor in breast cancer cells to stimulate...
Article
Full-text available
Within heterogeneous tumors, cancer stem cell (CSC) populations exhibit the greatest tumor initiation potential, promote metastasis, and contribute to therapy resistance. For breast cancer specifically, CSCs are identified by CD44highCD24low cell surface marker expression and increased aldehyde dehydrogenase activity. In general, bulk breast tumor...
Article
Full-text available
In vitro cell culture is traditionally performed within two-dimensional (2D) environments, providing a quick and cheap way to study cell properties in a laboratory. However, 2D systems differ from the in vivo environment and may not mimic the physiological cell behavior realistically. For instance, 2D culture models are thought to induce cancer ste...
Article
Full-text available
Background Breast cancer stem cells (BCSCs) are resistant to standard therapies, facilitate tumor dissemination, and contribute to relapse and progression. Super-enhancers are regulators of stemness, and BET proteins, which are critical for super-enhancer function, are a potential therapeutic target. Here, we investigated the effects of BET protein...
Article
Full-text available
Scientific evidence supports the early deregulation of epigenetic profiles during breast carcinogenesis. Research shows that cellular transformation, carcinogenesis, and stemness maintenance are regulated by epigenetic-specific changes that involve microRNAs (miRNAs). Dietary bioactive compounds such as blueberry polyphenols may modulate susceptibi...