Prediction of human RCK structure. (A) Western blot analysis of p‐MST1, MST1, HuR, Rck, DCP2, and Actb in cell lysates derived from pre‐adipocytes (Day 0) and mature adipocytes (Day 8) from Student's t‐test. (B) RT‐qPCR levels of Fabp4 and Gcg mRNAs normalized to Gapdh mRNA purified from Rck or IgG immunopellets from cell lysates of precursor and mature adipocytes. Data are expressed as mean ± SD of three independent experiments. N = 3, **p < 0.01, NS, not significant from Student's t‐test. (C) Human RCK structure predicted using the AlphaFold database provided by Deepmind and EMBL. The colors indicate the accuracy of the model based on a per‐residue confidence score (pLDDT) between 0 and 100. Blue represents pLDDT values over 90 (Very high). Sky blue indicates pLDDT values between 70 and 90 (Confident). Yellow color represents pLDDT values between 50 and 60 (Low). Deep orange color indicates the pLDDT values below 50 (Very low). The phosphorylation sites, Thr 36 and Thr 75, are indicated by the red circles based on the direction of the side chain. (D) mRNA (RT‐qPCR, right) and protein (Western blot, left) levels of Fabp4, Gcg, and Actb mRNAs and proteins from pre‐adipocytes (Day 0) and mature adipocytes (Day 8) after transfection of the Rck shRNA or control. Values are expressed as mean ± SD of three independent experiments (p < 0.001, Student's t‐test). N = 3, ***p < 0.001, NS, not significant from Student's t‐test.