Figure - available from: Mitochondrial DNA Part B
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The Pygmy corydoras Corydoras pygmaeus Knaack, 1966, is the smallest member of the genus Corydoras, belonging to the family Callichthyidae and order Siluriformes. The complete mitochondrial genome of C. pygmaeus was sequenced and assembled using next-generation sequencing technology, and phylogenetically compared with those of other species of this...
Citations
: Background/Objectives: In this study, we report the complete mitochondrial genome sequence of Hoplisoma concolor Weitzman, 1961 (Siluriformes: Callichthyidae), a callichthyid catfish. Methods: DNA sequencing was performed to obtain its complete mitogenome using the HiSeq platform. To assess the phylogenetic relationships, maximum-likelihood and Bayesian inference phylogenetic trees were constructed using two ribosomal RNA (rRNA) genes and all protein-coding sequences (PCGs) concatenated from the H. concolor mitogenome, along with 31 other Siluriformes mitogenomes. Results: The complete mitogenome of H. concolor is 16,579 base pairs in length, with a nucleotide composition of 32.2% A, 26.0% T, 15.3% G, and 26.5% C. It contains 13 PCGs, 22 transfer RNA genes, and 2 rRNA genes. Phylogenetic analysis based on all PCGs and two rRNAs of the complete mitogenome confirms H. concolor as a sister species of H. panda within the subfamily Corydoradinae. In addition, intergenic sequences between atp6 and cox3 of 21 species of Corydoradinae provide further support for their phylogenetic relationship. Conclusions: Given the lack of detailed descriptions regarding the length and nucleotide composition of these intergenic sequences, our study contributes valuable insights into the genetic diversity and evolutionary complexity of Callichthyidae.
The complete mitogenome of four Scleromystax barbatus populations distributed along the species natural range was reconstructed, comprising the first study with this genus and the second with Corydoradinae specimens sampled from their natural environment and deposited in permanent collections. The mitogenome of S. barbatus is a circular molecule of 16,694 base pairs (bp) comprising 37 genes, 22 of which are tRNA, two are rRNA, 13 are protein-coding genes and one control region (D-loop). An 18-nucleotide insertion sequence was found between the ATPase subunit 6 and COIII genes. Most genes are encoded on the heavy strand, while the ND6 and eight tRNAs are found on the light strand. Phylogenetic analyses using other available Callichthyidae mitogenomes confirmed the monophyly of Callichthyinae and Corydoradinae and indicate that S. barbatus populations form a separate and more closely related branch of Corydoras nattereri + Corydoras paleatus.
