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Passionfruit vines infected with Fusarium wilt caused by Fusarium oxysporum f. sp. passiflorae.  

Passionfruit vines infected with Fusarium wilt caused by Fusarium oxysporum f. sp. passiflorae.  

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Citations

... The vegetative propagation method (through stem cuttings) is most popular all over the world to maintain all essential superior characters of the genotype like disease resistance, size of fruit, juice content, time of maturity, and so forth. But this vine is affected by several viral, bacterial, and fungal diseases which caused heavy loss to the growers [8]. The vegetative propagation method causes the carry-over of disease-causing microorganisms from mother plant to the next generation [9]. ...
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A procedure for rapid clonal propagation of Passiflora edulis Sims. f. flavicarpa Deg. (Passifloraceae) has been developed in this study. Nodal explants were sterilized with 0.1% HgCl2 and inoculated on Murashige and Skoog (MS) basal medium. The addition of 2.0 mgL(-1) 6-benzylaminopurine (BAP) to MS medium caused an extensive proliferation of multiple shoots (8.21 ± 1.13) primordial from the nodal meristems. Subculturing of these multiple shoots on the MS medium augmented with 1.0 mgL(-1) of each BAP and Kinetin (Kin) was successful for the multiplication of the shoots in vitro with maximum numbers of shoots (25.73 ± 0.06) within four weeks of incubation. Shoots were rooted best (7.13 ± 0.56 roots/shoots) on half strength MS medium supplemented with 2.0 mgL(-1) indole-3 butyric acid (IBA). All in vitro regenerated shoots were rooted by ex vitro method, and this has achieved 6-7 roots per shoot by pulsing of cut ends of the shoots using 200 as well as 300 mgL(-1) IBA. The plantlets were hardened in the greenhouse for 4-5 weeks. The hardened plantlets were shifted to manure containing nursery polybags after five weeks and then transferred to a sand bed for another four weeks for acclimatization before field planting with 88% survival rate.
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