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PCR protocol and conditions, including time and temperature of all steps for ADHEX1 and ADHEX2 primers.
Source publication
Public swimming pools, if not treated well could work as a reservoir of many microorganisms that cause infections among swimmers. Conjunctivitis is one of those common infections that resulted from microbial and non-microbial agents, microbial conjunctivitis caused by viral (mainly Human adenovirus HAdVs) and bacterial infections. This study aims t...
Contexts in source publication
Context 1
... was carried out by (Gradient thermal cycler Alpha Cycler PCRmax series). PCR conditions (steps, time, and temperature) for the first primer performed as described (see Table 2). One microliter of the first-round PCR was transferred to a nested PCR mixture containing: 31 µl nuclease-free water, 5 µl 10X PCR buffer, 8 µl of MgCl2, 1.5 µl of 10 mmol dNTP mixes, 0.5 µl primer ADHEX2F (100 pmol), 0.5 µl primer ADHEX2R 100 pmol and 2.5 U Taq polymerase (Promega). ...Context 2
... microliter of the first-round PCR was transferred to a nested PCR mixture containing: 31 µl nuclease-free water, 5 µl 10X PCR buffer, 8 µl of MgCl2, 1.5 µl of 10 mmol dNTP mixes, 0.5 µl primer ADHEX2F (100 pmol), 0.5 µl primer ADHEX2R 100 pmol and 2.5 U Taq polymerase (Promega). PCR condition (steps, time, and temperature) for the second primer was carried out as described (see Table 2). ...Context 3
... was carried out by (Gradient thermal cycler Alpha Cycler PCRmax series). PCR conditions (steps, time, and temperature) for the first primer performed as described (see Table 2). One microliter of the first-round PCR was transferred to a nested PCR mixture containing: 31 µl nuclease-free water, 5 µl 10X PCR buffer, 8 µl of MgCl2, 1.5 µl of 10 mmol dNTP mixes, 0.5 µl primer ADHEX2F (100 pmol), 0.5 µl primer ADHEX2R 100 pmol and 2.5 U Taq polymerase (Promega). ...Context 4
... microliter of the first-round PCR was transferred to a nested PCR mixture containing: 31 µl nuclease-free water, 5 µl 10X PCR buffer, 8 µl of MgCl2, 1.5 µl of 10 mmol dNTP mixes, 0.5 µl primer ADHEX2F (100 pmol), 0.5 µl primer ADHEX2R 100 pmol and 2.5 U Taq polymerase (Promega). PCR condition (steps, time, and temperature) for the second primer was carried out as described (see Table 2). ...
