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- Fusobacterium nucleatum promotes tumor progression in KRAS p.G12D-mutant colorectal cancer by binding to DHX15

P. distasonis alleviates the F. nucleatum-mediated CRC progression a Representative images of the colons of Villin-Cre/KrasG12D+/⁻ mice treated with AOM/DSS and F. nucleatum or/and P. distasonis, n = 3 per group. b Representative H&E stainings of the colons treated in (a), n = 3 per group, scale bar: 20 μm. c–e Tumor numbers, tumor loads, and size of Villin-Cre/KrasG12D+/⁻ mice treated in (a). Significant differences are indicated: one-way ANOVA with Bonferroni’s multiple comparison test, n = 3 per group, data are presented as the mean ± SEM. f FISH detection of F. nucleatum (red) and P. distasonis (green) in colonic organoids derived from KRAS WT and KRAS p.G12D CRC patients treated by F. nucleatum or/and P. distasonis, scale bar: 50 μm. g Statistical analysis of the results in (f). Significant differences are indicated: two-tailed Student’s t-test, n = 5 per group, data are presented as the mean ± SEM. h Representative immunostainings of Ki67 in colonic organoids derived from KRAS WT and KRAS p.G12D CRC patients treated by F. nucleatum or/and P. distasonis, scale bar: 50 μm. i Statistical analysis of the results in (h). Significant differences are indicated: one-way ANOVA with Bonferroni’s multiple comparison test, n = 5 per group, data are presented as the mean ± SEM. Data are representative of two independent experiments. Source data are provided as a Source Data file.
P. distasonis alleviates the F. nucleatum-mediated CRC progression a Representative images of the colons of Villin-Cre/KrasG12D+/⁻ mice treated with AOM/DSS and F. nucleatum or/and P. distasonis, n = 3 per group. b Representative H&E stainings of the colons treated in (a), n = 3 per group, scale bar: 20 μm. c–e Tumor numbers, tumor loads, and size of Villin-Cre/KrasG12D+/⁻ mice treated in (a). Significant differences are indicated: one-way ANOVA with Bonferroni’s multiple comparison test, n = 3 per group, data are presented as the mean ± SEM. f FISH detection of F. nucleatum (red) and P. distasonis (green) in colonic organoids derived from KRAS WT and KRAS p.G12D CRC patients treated by F. nucleatum or/and P. distasonis, scale bar: 50 μm. g Statistical analysis of the results in (f). Significant differences are indicated: two-tailed Student’s t-test, n = 5 per group, data are presented as the mean ± SEM. h Representative immunostainings of Ki67 in colonic organoids derived from KRAS WT and KRAS p.G12D CRC patients treated by F. nucleatum or/and P. distasonis, scale bar: 50 μm. i Statistical analysis of the results in (h). Significant differences are indicated: one-way ANOVA with Bonferroni’s multiple comparison test, n = 5 per group, data are presented as the mean ± SEM. Data are representative of two independent experiments. Source data are provided as a Source Data file.
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