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Overview of the setup on the instrument deck of the Hamilton MicroLab STAR liquid-handling platform.
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Objective
We aimed to find new methods to detect and quantify hemolysis and icterus which may cause assay biases. These methods need to determine each of these interferents in the presence of various other interferents. They also need to have less stringent requirements in development and implementation than those conventional analyzers currently m...
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Objective
Hemolysis, icterus, and lipemia (HIL) of blood samples have been a concern in hospitals because they reflect pre-analytical processes' quality control. However, very few studies investigate the influence of patients' gender, age, and department, as well as sample-related turnaround time, on the incidence rate of HIL in fasting serum bioch...
The aim of this study was to determine the rate of hemolyzed specimens sent to our laboratory for coagulation testing, assess the interference of hemolysis on coagulation for patients without anticoagulant therapy and to determine the reference intervals for PT, INR and aPTT for our laboratory in order to test our own limitations.
Methods : To dete...
Citations
... Icterus manifests as a broad absorbance peak around 460 nm, which sometimes interferes with other absorbance peaks due to hemoglobin. In lipemia, attenuation of collimated light occurs in a wide range of wavelengths from UV to visible, slowly decreasing with the wavelength [19]. Strong water absorption affects the transmittance at lengths close to infrared (IR) and far IR. ...
... 1.3511 ± 0.0 slowly decreasing with the wavelength [19]. Strong water absorption affects the tance at lengths close to infrared (IR) and far IR. ...
Today, visual classification of the degree of lipemia in blood samples is frequently performed in clinical laboratories. However, achieving standardization of this classification at low cost and with fewer resources is an objective that is still under development. In this work, a comparison is made between the visual inspection and optical measurements of blood plasma for quantifying lipemia. The plasma refractive index’s real part was measured using an Abbe refractometer and transmittance measurements were made at a 589 nm wavelength and wavelengths ranging from 320 to 1100 nm in the spectral region, respectively. Taking the slope of the transmittance spectrum at two specific wavelengths, it is possible to establish a more standardized selection criterion and implement it quickly using low-cost optical devices. Furthermore, using the proposed transmittance-spectrum-slope method, statistically significant differences (p < 0.05) were found between healthy blood samples and lipemia 1, 2, 3, and 4. However, there were no statistical differences between lipemia 1 and 2.
... During hospitalization CT scan was performed with evidence of thorax progressive disease, pleural and diaphragmatic infiltration and hepatic localizations. Before oncologic evaluation, the patient required new hospitalization because of febrile severe acute respiratory failure due to pleural effusion: no infective causes were detected neither hemolysis signs were identifiable [8]. Few days after patient died (Table 1). ...
Sonothrombolysis with recombinant tissue plasminogen activator (rtPA) and microbubbles has been widely studied to enhance thrombolytic potential. Here, we report different sonothrombolysis strategy in nanoparticles using microbubbles cavitation. We found that different particles in shape exhibited different reactivity toward the cavitation, leading to a distinct sonothrombolytic potential. Two different gold nanoparticles in shape were functionalized with the rtPA: rtPA-functionalized gold nanospheres (NPt) and gold nanostars (NSt). NPt could not accelerate the thrombolytic potential with a sole acoustic stimulus. Importantly, NSt enhanced the potential with acoustic stimulus and microbubble-mediated cavitation, while NPt were not reactive to cavitation. Coadministration of NSt and microbubbles resulted in a dramatic reduction of the infarcts in a photothrombotic model and recovery in the cerebral blood flow. Given the synergistic effect and in vivo feasibility of this strategy, cavitation-assisted sonothrombolysis by asymmetrical NSt might be useful for treating acute ischemic stroke.
The ability to perform laboratory testing near the patient and with smaller blood volumes would benefit patients and physicians alike. We describe our design of a miniaturized clinical laboratory system with 3 components: a hardware platform (ie, the miniLab) that performs pre-analytical and analytical processing steps using miniaturized sample manipulation and detection modules, an assay-configurable cartridge that provides consumable materials and assay reagents, and a server that communicates bi-directionally with the miniLab to manage assay-specific protocols and analyze, store, and report results (ie, the virtual analyzer). The miniLab can detect analytes in blood using multiple methods, including molecular diagnostics, immunoassays, clinical chemistry, and hematology. Analytical performance results show that our qualitative Zika virus assay has a limit of detection of 55 genomic copies/mL. For our anti-herpes simplex virus type 2 immunoglobulin G, lipid panel, and lymphocyte subset panel assays, the miniLab has low imprecision, and method comparison results agree well with those from the United States Food and Drug Administration-cleared devices. With its small footprint and versatility, the miniLab has the potential to provide testing of a range of analytes in decentralized locations. This article is protected by copyright. All rights reserved.
