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Overview of molecular biology methods and their respective “Pros and Cons” in investigating microbial diversity and biogeochemical processes. Emphasis is placed on exploring microbial functions linked to greenhouse gas (GHG) emissions in rewetted peatlands. (PCR polymerase chain reation)

Overview of molecular biology methods and their respective “Pros and Cons” in investigating microbial diversity and biogeochemical processes. Emphasis is placed on exploring microbial functions linked to greenhouse gas (GHG) emissions in rewetted peatlands. (PCR polymerase chain reation)

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Restoration of drained peatlands through rewetting has recently emerged as a prevailing strategy to mitigate excessive greenhouse gas emissions and re-establish the vital carbon sequestration capacity of peatlands. Rewetting can help to restore vegetation communities and biodiversity, while still allowing for extensive agricultural management such...

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... However, the study also confirms that long-term GHG balance datasets and analyses are too scarce, and that lateral losses of dissolved and particulate organic matter and associated GHG fluxes from streams are often overlooked, to derive a comprehensive picture of drainage-and restorationinduced changes in peatland GHG fluxes (Fig. 1). An additional review synthesizes the research highlighting the pivotal role of soil microbial communities in regulating carbon and nutrient cycling within rewetted peatlands (Gios et al. 2023). This encompasses an examination of molecular biology techniques designed to better understand the biogeochemical processes linked to GHG fluxes. ...