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Overview of Joint-snhmC-seq. The figure demonstrates the concurrent profiling of 5mC and 5hmC at the single-cell level.
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Overview of the joint profiling technologies of 5mC and 5hmC.
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Context 1
... methods used in Joint-snhmC-seq involve a combination of technologies and processes to simultaneously profile 5mC and 5hmC in single cells 38 (Fig. 3). First, lysed individual cells/ Fig. 1 Overview of dyad-seq. This figure illustrates the schematic and sequencing features of the dyad-seq for combined detection of 5mC and ...
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Citations
... Conversely, 5hmC counteracts these effects by being concentrated in specific genomic regions, regulating gene activation, and preventing excessive gene silencing due to abnormal DNA methylation. Both modifications interact to maintain the dynamic balance between gene activation and silencing [24]. In conjunction with our research, we observed dynamic changes in the modification level of DNA demethylation-modified 5mC in muscle satellite cells. ...
To investigate prenatal muscle satellite cell (MuSC) development and the associated epigenetic modifications in yak. Here, we conducted morphological and protein co-localization analyses of fetal longissimus dorsi muscle at various developmental stages using histology and immunofluorescence staining methods. Our study observed that primary muscle fibers began forming at 40 days of gestation, fully developed by 11 weeks, and secondary muscle fibers were predominantly formed by around 105 days. Throughout development, MuSCs were mainly located between the muscle fiber membrane and the basement membrane, acting as a reserve for the stem cell pool. MuSCs appeared within myotubes only during critical phases of primary and secondary muscle fiber formation. The proliferation of MuSCs gradually decreases until birth. MuSCs with 5mC modification show a trend of increasing first and then decreasing. MuSCs with 5hmC modification also present a dynamic change trend. The 41st day and 11th week are the critical periods for the changes of both. From the 11th week to around the 110th day of gestation, the modification effect of histone H3K4me3 is crucial for MuSCs during the development of the fetal longissimus dorsi muscle. Combined, our data identify key time points for yak fetal skeletal muscle growth and development and demonstrate that DNA methylation and histone modifications in MuSCs are closely related to this process, offering a valuable basis for future research into the molecular mechanisms underlying yak muscle development.
In the era of precision oncology, identifying abnormal genetic and epigenetic alterations has transformed the way cancer is diagnosed, managed, and treated. 5-hydroxymethylcytosine (5hmC) is an emerging epigenetic modification formed through the oxidation of 5-methylcytosine (5mC) by ten-eleven translocase (TET) enzymes. DNA hydroxymethylation exhibits tissue- and cancer-specific patterns and is essential in DNA demethylation and gene regulation. Recent advancements in 5hmC detection methods and the discovery of 5hmC in cell-free DNA (cfDNA) have highlighted the potential for cell-free 5hmC as a cancer biomarker. This review explores the current and emerging techniques and applications of DNA hydroxymethylation in cancer, particularly in the context of cfDNA.
