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Optimization of dahlia petals protoplast yield and viability. a Effect of treatments on the separation of dahlia
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Dahlia ( Dahlia sp. ), a species of the Asteraceae family, is widely cultivated in China and exhibits seasonal blooming. The main challenges associated with this species are low cross-breeding efficiency and slow breeding. In this study, dahlia leaves and petals were employed as test materials to ascertain the optimal conditions for protoplast isol...
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Context 1
... of dahlia petal tissues by shredding, blocking, and crushing methods, the 248 number of protoplasts and their viability were counted after 4 h of enzyme digestion. The shredding 249 method yielded the highest number of protoplasts, at 3.67 × 10⁵ per millilitre, which was 2 to 11 times 250 higher than that of the crushing and blocking methods (Fig. 7a). The viability of dahlia protoplasts was 251 found to be greater than 80%, although the yield was lower at 2 and 4 hours. The viability was ...