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OE-CEACAM5 increased CG malignant progression. (A-B) Detected the impact of OE-CEACAM5 on CEACAM5 expression in GC cells using Western blot. (C) Assessed the impact of OE-CEACAM5 on CEACAM5 mRNA levels in GC cells by RT-qPCR. (D) Utilized CCK-8 assay to monitor the cell proliferation following transfection with OE-CEACAM5. (E-F) The migration ability of GC cells was enhanced after OE-CEACAM5. (G-H) The amount of invasion cells was performed by transwell assay. (I-J) Flow cytometry showed the apoptosis of GC cells was reduced after OE-CEACAM5. (K-L) Examined the impact of transfected OE-CEACAM5 on apoptosis-related proteins in GC cells. (M-P) The EMT-related protein levels following transfection with OE-CEACAM5 were detected by Western blot.
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Background: There are some reports indicating that carcinoembryonic antigen associated cell adhesion molecule 5 (CEACAM5) can act as a diagnostic indicator or molecular marker for GC, but its upstream regulatory pathway still needs to be explored. microRNAs (miRNAs) regulate gene expression at the translation level and fulfil a crucial role in GC p...
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... impact of CEACAM5 expression in GC cells and found that OE-CEACAM5 effectively increased CEACAM5 protein and mRNA levels in GC cells using Western blot and RT-qPCR ( Figure 5A-5C). OE-CEACAM5 effectively increased GC cell proliferation, migration, invasion, and decreased apoptosis ( Figure 5D-5J). ...
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... impact of CEACAM5 expression in GC cells and found that OE-CEACAM5 effectively increased CEACAM5 protein and mRNA levels in GC cells using Western blot and RT-qPCR ( Figure 5A-5C). OE-CEACAM5 effectively increased GC cell proliferation, migration, invasion, and decreased apoptosis ( Figure 5D-5J). After transfection with OE-CEACAM5, there was a substantial reduction in bax and a noticeable elevation in bcl2 in GC cells ( Figure 5K-5L). ...
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... effectively increased GC cell proliferation, migration, invasion, and decreased apoptosis ( Figure 5D-5J). After transfection with OE-CEACAM5, there was a substantial reduction in bax and a noticeable elevation in bcl2 in GC cells ( Figure 5K-5L). Figure 5M-5P showed that E-cadherin level were declined, whereas N-cadherin and Vimentin levels were risen in GC cells of the OE-CEACAM5 group. ...
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... transfection with OE-CEACAM5, there was a substantial reduction in bax and a noticeable elevation in bcl2 in GC cells ( Figure 5K-5L). Figure 5M-5P showed that E-cadherin level were declined, whereas N-cadherin and Vimentin levels were risen in GC cells of the OE-CEACAM5 group. These findings suggested that OE-CEACAM5 could effectively promote GC cell proliferation, migration, invasion, and EMT. ...