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Normal human lymphocyte and CLL cancerous cell treated with activated parasporal protein produced by isolates of B. thuringiesis. A-Normal human lymphocytes treated for 12 hours with activated parasporal protein of BtA1 and BtA5. B-Normal human lymphocytes treated for one hours either activated parasporal protein of BtA2, 3, 4 or 6. C-CLL cancerous cells treated for 12 hours with either activated parasporal protein of BtA1 or BtA5. D-Cell lyses and ruptured of CLL cancerous cells treated for 12 hours with activated parasporal protein of BtA2, 3, 4 and 6.
Source publication
Six isolates of Bacillus thuringiensis were isolated from Iraqi soil characterized as non- insecticidal and non- hemolytic parasporal inclusion proteins. Bacterial isolates were propagated on nutrient broth. Then, the parasporal inclusion proteins were extracted and processed with proteinase K and trypsin. The major protein segments produced of 64K...
Contexts in source publication
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... other four isolates (BtA2, BtA3, BtA4 and BtA6) proteins showed cytocidal activity and hemolytic activity and not discriminates between normal and cancerous cells (table 5). No remarks was appears on normal lymphocytes as a results of treatment with activated parasporal proteins of BtA1 or BtA5 (Fig.3A). However, activated parasporal protein of BtA2 and BtA3 showed extensive lytic effect on normal lymphocytes (Fig. 3 B). ...
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... cytocidal activity and hemolytic activity and not discriminates between normal and cancerous cells (table 5). No remarks was appears on normal lymphocytes as a results of treatment with activated parasporal proteins of BtA1 or BtA5 (Fig.3A). However, activated parasporal protein of BtA2 and BtA3 showed extensive lytic effect on normal lymphocytes (Fig. 3 B). While, treatment of cancerous cell (CLL) with either BtA1 or BtA5 activated parasporal proteins showed remarkable cytopathic effect representing by granulation of cytoplasm, nucleus regeneration and obvious cell ballooning (Fig. 3C). Namba et al. 2003). Also, Krishnan et al. (2017) found that the toxin induce a rapid and significant ...
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... However, activated parasporal protein of BtA2 and BtA3 showed extensive lytic effect on normal lymphocytes (Fig. 3 B). While, treatment of cancerous cell (CLL) with either BtA1 or BtA5 activated parasporal proteins showed remarkable cytopathic effect representing by granulation of cytoplasm, nucleus regeneration and obvious cell ballooning (Fig. 3C). Namba et al. 2003). Also, Krishnan et al. (2017) found that the toxin induce a rapid and significant decrease in metabolic activity, depletion of adenosine triphosphate (ATP) and cell swelling with membrane damage. Mizuki et al. (2001) found as a results of screening 1744 B. Thuringiesis strains, parasporal protein has highly ...
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... other four isolates (BtA2, BtA3, BtA4 and BtA6) proteins showed cytocidal activity and hemolytic activity and not discriminates between normal and cancerous cells (table 5). No remarks was appears on normal lymphocytes as a results of treatment with activated parasporal proteins of BtA1 or BtA5 (Fig.3A). However, activated parasporal protein of BtA2 and BtA3 showed extensive lytic effect on normal lymphocytes (Fig. 3 B). ...
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... cytocidal activity and hemolytic activity and not discriminates between normal and cancerous cells (table 5). No remarks was appears on normal lymphocytes as a results of treatment with activated parasporal proteins of BtA1 or BtA5 (Fig.3A). However, activated parasporal protein of BtA2 and BtA3 showed extensive lytic effect on normal lymphocytes (Fig. 3 B). While, treatment of cancerous cell (CLL) with either BtA1 or BtA5 activated parasporal proteins showed remarkable cytopathic effect representing by granulation of cytoplasm, nucleus regeneration and obvious cell ballooning (Fig. 3C). Namba et al. 2003). Also, Krishnan et al. (2017) found that the toxin induce a rapid and significant ...
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... However, activated parasporal protein of BtA2 and BtA3 showed extensive lytic effect on normal lymphocytes (Fig. 3 B). While, treatment of cancerous cell (CLL) with either BtA1 or BtA5 activated parasporal proteins showed remarkable cytopathic effect representing by granulation of cytoplasm, nucleus regeneration and obvious cell ballooning (Fig. 3C). Namba et al. 2003). Also, Krishnan et al. (2017) found that the toxin induce a rapid and significant decrease in metabolic activity, depletion of adenosine triphosphate (ATP) and cell swelling with membrane damage. Mizuki et al. (2001) found as a results of screening 1744 B. Thuringiesis strains, parasporal protein has highly ...
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... other four isolates (BtA2, BtA3, BtA4 and BtA6) proteins showed cytocidal activity and hemolytic activity and not discriminates between normal and cancerous cells (table 5). No remarks was appears on normal lymphocytes as a results of treatment with activated parasporal proteins of BtA1 or BtA5 (Fig.3A). However, activated parasporal protein of BtA2 and BtA3 showed extensive lytic effect on normal lymphocytes (Fig. 3 B). ...
Context 8
... cytocidal activity and hemolytic activity and not discriminates between normal and cancerous cells (table 5). No remarks was appears on normal lymphocytes as a results of treatment with activated parasporal proteins of BtA1 or BtA5 (Fig.3A). However, activated parasporal protein of BtA2 and BtA3 showed extensive lytic effect on normal lymphocytes (Fig. 3 B). While, treatment of cancerous cell (CLL) with either BtA1 or BtA5 activated parasporal proteins showed remarkable cytopathic effect representing by granulation of cytoplasm, nucleus regeneration and obvious cell ballooning (Fig. 3C). Namba et al. 2003). Also, Krishnan et al. (2017) found that the toxin induce a rapid and significant ...
Context 9
... However, activated parasporal protein of BtA2 and BtA3 showed extensive lytic effect on normal lymphocytes (Fig. 3 B). While, treatment of cancerous cell (CLL) with either BtA1 or BtA5 activated parasporal proteins showed remarkable cytopathic effect representing by granulation of cytoplasm, nucleus regeneration and obvious cell ballooning (Fig. 3C). Namba et al. 2003). Also, Krishnan et al. (2017) found that the toxin induce a rapid and significant decrease in metabolic activity, depletion of adenosine triphosphate (ATP) and cell swelling with membrane damage. Mizuki et al. (2001) found as a results of screening 1744 B. Thuringiesis strains, parasporal protein has highly ...
