Negative mode LC-MS/MS of AJHW: (A) extracted ion chromatogram of AJHW, (B) total ion chromatogram, where the highlighted section corresponds to the mass value of hirsutanonol, and (C) total ion chromatogram, where the highlighted section corresponds to the mass value of hirsutenone.

Negative mode LC-MS/MS of AJHW: (A) extracted ion chromatogram of AJHW, (B) total ion chromatogram, where the highlighted section corresponds to the mass value of hirsutanonol, and (C) total ion chromatogram, where the highlighted section corresponds to the mass value of hirsutenone.

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This study investigates the effects of pilot scale Alnus japonica hot water extract (AJHW) on muscle loss and muscle atrophy. Building on previous in vitro studies, in vivo experiments were conducted to evaluate muscle strength, mass, fiber size, protein synthesis, and antioxidant activity. The results showed that AJHW significantly restored muscle...

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