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- Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Norway: Evaluation of a PCR test targeting the chromosomal flaB gene

Multiple sequence alignment of target, primer and probe sequences. Dots indicated identity to the reference sequence. Hyphens indicate regions where no sequence information is available. The following IUPAC ambiguity codes are used: Y = T/C; R = A/G; M = A/C; S = G/C; H = A/C/T; K = G/T; D = A/G/T; B = C/T/G. Variant positions are indicated by lower case letters in the consensus and primer/probe sequences. Variants accommodated by G:T basepairing or degeneracy of the primer/probe sequence are highlighted in green in the target sequence. Variants not so accommodated are highlighted in red. Primer and probe sequences are highlighted in blue. Unless otherwise noted, sequences are written 5’ – 3’. (Color figure online)
Multiple sequence alignment of target, primer and probe sequences. Dots indicated identity to the reference sequence. Hyphens indicate regions where no sequence information is available. The following IUPAC ambiguity codes are used: Y = T/C; R = A/G; M = A/C; S = G/C; H = A/C/T; K = G/T; D = A/G/T; B = C/T/G. Variant positions are indicated by lower case letters in the consensus and primer/probe sequences. Variants accommodated by G:T basepairing or degeneracy of the primer/probe sequence are highlighted in green in the target sequence. Variants not so accommodated are highlighted in red. Primer and probe sequences are highlighted in blue. Unless otherwise noted, sequences are written 5’ – 3’. (Color figure online)
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