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Qualitative and quantitative morphological characterization in different growth stages of Nyctanthes arbor-tristis L. fruits and seeds were investigated. Capsules are compressed, two celled, green, cordate to round or elliptical with one flattened seed in each half. Both LM and SEM study were conducted to gather micromorphological features of matur...
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... seeds were flattened, obovate, veined reticulate, and two in number in each fruit. The size of matured seed was 11-14 × 8-11.5 mm² (Fig. 3L, 4D, 4E; Table 2). Elongated fruits showed slightly longer seed and less width than those of cordate shaped fruits, but both types showed a more or less similar size ratio of fruit and seed. ...Context 2
... fruits showed slightly longer seed and less width than those of cordate shaped fruits, but both types showed a more or less similar size ratio of fruit and seed. The seed colour gradually changed from greenish-white to brown (Table 2). Seeds can be easily separated when the fruit gets fully matured and ripened. ...Context 3
... seeds were flattened, obovate, veined reticulate, and two in number in each fruit. The size of matured seed was 11-14 × 8-11.5 mm² (Fig. 3L, 4D, 4E; Table 2). Elongated fruits showed slightly longer seed and less width than those of cordate shaped fruits, but both types showed a more or less similar size ratio of fruit and seed. ...Context 4
... fruits showed slightly longer seed and less width than those of cordate shaped fruits, but both types showed a more or less similar size ratio of fruit and seed. The seed colour gradually changed from greenish-white to brown (Table 2). Seeds can be easily separated when the fruit gets fully matured and ripened. ...Context 5
... seeds were flattened, obovate, veined reticulate, and two in number in each fruit. The size of matured seed was 11-14 × 8-11.5 mm² (Fig. 3L, 4D, 4E; Table 2). Elongated fruits showed slightly longer seed and less width than those of cordate shaped fruits, but both types showed a more or less similar size ratio of fruit and seed. ...Context 6
... fruits showed slightly longer seed and less width than those of cordate shaped fruits, but both types showed a more or less similar size ratio of fruit and seed. The seed colour gradually changed from greenish-white to brown (Table 2). Seeds can be easily separated when the fruit gets fully matured and ripened. ...Similar publications
Lamb’s-ear (Stachys byzantina L.) serves as valuable medicinal plant mainly because of its pharmaceutical phyto-metabolites like linalool. Polyploidy induction has been known as an effective approach to enhance phyto-medicines content in various medicinal plants. To fulfill the production of S. byzantin with higher biomass and more secondary metabo...
Citations
... The new populations of J. azoricum, J. adenophyllum and J. malabaricum were discovered and collected from natural habitat in the districts Jalpaiguri and Paschim Burdwan of West Bengal (India) (Fig. 1). Observations of leaf epidermis and venation were done under a trinocular upright microscope (Magnus, 528013) and a stereo zoom light microscope (Olympus, SZ2-ILST 8A11112) and dry leaf samples were studied by scanning electron microscopy (SEM, JEOL JSM-IT100) following different methods described in previous studies (Hickey 1973;Barthlott 1981;Modak and Chowdhury 2021;Moura et al. 2024) with a few modifications. Prior to microscopic examination, raw leaves were immersed in an aqueous solution of NaOH (5%) for dechlorophyllization followed by washing and staining for observation of venation category. ...
Jasmine is widely used in the aromatic industries and the pharmacological, phytochemical and cosmetic sectors. Applied studies of wild plants with proper identification are becoming increasingly common globally. A detailed taxonomic description of three wild jasmines is given, including domatia characters, foliar epidermal characteristics (crystals, epidermal cell wall patterns, trichomes) and venation types. New localities were discovered, which significantly extended their distribution in India. A taxonomic key for the identification of the study species and related, potentially confusable Indian members of Jasminum sect. Unifoliolata and sect. Trifoliolata is given. The morphological identification is supported by molecular attributes. The molecular phylogeny of the species studied and their closest taxa based on the Internal Transcribed Spacer (ITS) region of nuclear-ribosomal DNA is provided. Species-specific nucleotide variations are highlighted. Phylogenetic analyses revealed five major clades that were consistent with morphological characteristics. Jasminum azoricum and J. malabaricum are reported here from new sites in India beyond Western Ghats, while J. adenophyllum is reported here outside of Assam. The present work focused on the domatia identified in J. azoricum and J. flexile and key differentiation characters.
... Thus, from the given data, it is evident that the seed maturity is attained 90 days after flowering. Modak and Chowdhury (2021) studied the morphological changes in the fruits and seeds under Darjeeling climatic conditions and reported that the fruit maturity is attained four months after flowering [12] . ...
Jasmine is widely associated with aromatic applications, pharmaceuticals, phytochemicals and cosmetic industries. Application and proper identification of wild plants are becoming increasingly common globally. A detailed morphological description, domatia characters, foliar epidermal characteristics (crystal, epidermal cell wall pattern, trichome), venation types of three wild, strong aromatic Jasmines with their extended distribution are given here and also provided taxonomic key for differentiating them from related species. The morpho-taxonomic details with some additional key features and molecular characterization were performed here for proper identification. The molecular phylogeny of studied species and their closest taxa is detailed here based on the Internal Transcribed Spacer (ITS) consensus sequence of nuclear-ribosomal DNA and highlights species specific nucleotide variations. Jasminum azoricum L. and J . malabaricum Wight are documented to have extended distribution beyond Western Ghats (India), while J . adenophyllum Wall. ex C.B.Clarke is reported here outside of Assam to Peninsula Malaysia. Critically endangered species J . azoricum is resemblance with J . flexile Vahl, but can be distinguished by the total number of flowers (8–19) per inflorescence; absence of leaf articulation; particular secondary venation type; calyx teeth length; number of petals, sepals. The fruits of J . malabaricum , a species native to the Western Ghat, are consumed by local tribals of Burdwan (India).
This work was aimed to establish an effective approach for direct regeneration of Nyctanthes arbor-tristis L. using nodal segment. Amongst the different combinations of plant growth regulators (PGR's), the maximum number of shoots/explant (32.94 ± 0.86) with 3.14 ± 0.08 cm shoot length was attained on Murashige and Skoog (MS) medium fortified with 3.5 mg /L 6-benzyladenine (BA) and 0.3 mg/L concentration of 1-phenyl-3-(1, 2, 3- thiadiazol-5-yl)- urea (TDZ). Besides, nodal segments of Nyctanthes grown on MS medium were used to analyze the effect of biogenic AgNPs (0,2,4,6,8 and 10mg/L) on plant growth & multiplication in solid & suspension culture medium. After 35 days of in vitro shoot proliferation, the shoot number, shoot length, chlorophyll content, fresh & dry matter, guaiacol peroxidase (GPOX) and catalases enzyme (CAT) activities of plants were quantified. The administration of AgNPs (6 mg/L) along with the lower concentration of BA (1mg/L) could enhance shoot multiplication from (1.29 ± 0.14) to (25.17 ± 0.45), chlorophyll content as well as the GPOX, and CAT enzyme activities in suspension culture medium. Optimized rooting response (12.15 ± 0.63) was noted on the half-strength MS medium supplemented with 0.5 mg/L concentration of Indole acetic acid (IAA), The maximum in vitro raised plantlets was hardened & acclimatized in a ratio of 6:2:2:1(garden soil: cocopeat: vermiculite: pearlite) potting mixture. The regenerated plantlets showed 80% survival rate under greenhouse conditions. Out of twenty PCR-based RAPD (Random Amplified Polymorphic DNA) molecular markers, 8 primers produced a total of 350 scorable bands which showed 90–99% similarity with mother plant.
