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Malaria burden remains one of the major public health challenges in sub-Saharan Africa, Uganda inclusive. Uganda has the 3 rd highest global disease cases estimated to be 225 million and the 8 th highest level of deaths equivalent to 781,000 per year. Malaria remains a leading cause of morbidity and mortality in Uganda, accounting for 30-50% of out...
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... funestus mosquito density have been observed in Tororo district (unpublished data). Busia is also a site of very high malaria transmission [41,42], but unlike Tororo, Busia has received LLINs only (without IRS) for vector control. Malaria transmission patterns in Busia are stable and characteristic of a high transmission area [37,41]. ...
... Busia is also a site of very high malaria transmission [41,42], but unlike Tororo, Busia has received LLINs only (without IRS) for vector control. Malaria transmission patterns in Busia are stable and characteristic of a high transmission area [37,41]. The dominant malaria vectors in Busia are An. ...
Abstract Background Methods used to sample mosquitoes are important to consider when estimating entomologic metrics. Human landing catches (HLCs) are considered the gold standard for collecting malaria vectors. However, HLCs are labour intensive, can expose collectors to transmission risk, and are difficult to implement at scale. This study compared alternative methods to HLCs for collecting Anopheles mosquitoes in eastern Uganda. Methods Between June and November 2021, mosquitoes were collected from randomly selected households in three parishes in Tororo and Busia districts. Mosquitoes were collected indoors and outdoors using HLCs in 16 households every 4 weeks. Additional collections were done indoors with prokopack aspirators, and outdoors with pit traps, in these 16 households every 2 weeks. CDC light trap collections were done indoors in 80 households every 4 weeks. Female Anopheles mosquitoes were identified morphologically and Anopheles gambiae sensu lato were speciated using PCR. Plasmodium falciparum sporozoite testing was done with ELISA. Results Overall, 4,891 female Anopheles were collected, including 3,318 indoors and 1,573 outdoors. Compared to indoor HLCs, vector density (mosquitoes per unit collection) was lower using CDC light traps (4.24 vs 2.96, density ratio [DR] 0.70, 95% CIs 0.63–0.77, p
